The aim of this work was to determinate effect of naphthalene at different concentrations on morphological, physiological and some metabolic responses of pea seedlings. The quantification of naphthalene and its by-products were also recorded by Gas Chromatography/ Mass Spectrometry (GC / MS) in soil and in the different plant parts (roots, stems, leaves and fruit). In our controlled laboratory studies, plants exposed to naphthalene were able to efficiently grow and maintain their content of chlorophyll and carotenoids comparatively to the control plants. However, the pollutant slightly increased the amounts of fatty acid peroxides and strongly those of malonyldialdehyde, the product of lipid peroxidation. The glutathione S transferase activity was also increased for all concentrations used especially in leaves. Chromatograms showed that naphthalene has fallen sharply in the soil or even disappeared for the highest concentration from the second to third week. Furthermore, the removal ratio of 67% of the pollutant from the soil was distributed between two metabolites (ion 47 and ion 59) in the leaves for this same concentration in only three weeks of cultivation. In parallel, the amount of pollutant remained higher in unvegetated control soil. These results suggest that seedlings of pea (Pisum sativum L.) can remove naphthalene from contaminated soil and consequently have a high potential to be used as a promising candidate for the phytoremediation of naphthalene-contaminated soil.
Keywords: PAH; naphtalene; pea; phytoremediation; pollution.