Nuclear localization signal targeting to macronucleus and micronucleus in binucleated ciliate Tetrahymena thermophila

Genes Cells. 2018 Jul;23(7):568-579. doi: 10.1111/gtc.12602. Epub 2018 Jun 8.

Abstract

Ciliated protozoa possess two morphologically and functionally distinct nuclei: a macronucleus (MAC) and a micronucleus (MIC). The MAC is transcriptionally active and functions in all cellular events. The MIC is transcriptionally inactive during cell growth, but functions in meiotic events to produce progeny nuclei. Thus, these two nuclei must be distinguished by the nuclear proteins required for their distinct functions during cellular events such as cell proliferation and meiosis. To understand the mechanism of the nuclear transport specific to either MAC or MIC, we identified specific nuclear localization signals (NLSs) in two MAC- and MIC-specific nuclear proteins, macronuclear histone H1 and micronuclear linker histone-like protein (Mlh1), respectively. By expressing GFP-fused fragments of these proteins in Tetrahymena thermophila cells, two distinct regions in macronuclear histone H1 protein were assigned as independent MAC-specific NLSs and two distinct regions in Mlh1 protein were assigned as independent MIC-specific NLSs. These NLSs contain several essential lysine residues responsible for the MAC- and MIC-specific nuclear transport, but neither contains any consensus sequence with known monopartite or bipartite NLSs in other model organisms. Our findings contribute to understanding how specific nuclear targeting is achieved to perform distinct nuclear functions in binucleated ciliates.

Keywords: MacNLS; MicNLS; ciliate; histone H1; micronuclear linker histone; nuclear dimorphism; nuclear localization signal; nuclear transport.

MeSH terms

  • Active Transport, Cell Nucleus / physiology*
  • Amino Acid Sequence
  • Animals
  • Cell Nucleus / metabolism
  • Histones / genetics
  • Histones / metabolism
  • Macronucleus / physiology
  • Micronucleus, Germline / physiology
  • Nuclear Localization Signals / genetics
  • Nuclear Localization Signals / physiology*
  • Protein Domains / physiology
  • Protozoan Proteins / genetics
  • Tetrahymena thermophila / genetics
  • Tetrahymena thermophila / metabolism*

Substances

  • Histones
  • Nuclear Localization Signals
  • Protozoan Proteins