Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

Ira M Sigar; Justin H Schripsema; Kathleen A Kelly; Ashlesh K Murthy; Srikanth Manam; Kyle H Ramsey

doi:10.1186/s13104-018-3455-x

Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

BMC Res Notes. 2018 Jun 7;11(1):361. doi: 10.1186/s13104-018-3455-x.

Authors

Ira M Sigar¹, Justin H Schripsema², Kathleen A Kelly³, Ashlesh K Murthy⁴, Srikanth Manam⁴, Kyle H Ramsey²

Affiliations

¹ Microbiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern University, SH-323H, 555 31st Street, Downers Grove, IL, USA. isigar@midwestern.edu.
² Microbiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern University, SH-323H, 555 31st Street, Downers Grove, IL, USA.
³ Pathology and Laboratory Medicine, David Geffen School of Medicine, at UCLA, Los Angeles, CA, USA.
⁴ College of Veterinary Medicine, Midwestern University, Glendale, AZ, USA.

Abstract

Objective: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay.

Results: We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay.

Keywords: Cell culture; Chlamydia; Contamination; Mycoplasma; Plaque assay.

MeSH terms

Animals
Chlamydia*
Female
Genetic Techniques*
HeLa Cells
Humans
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Microbiological Techniques / methods*
Mycoplasma*
Polymerase Chain Reaction