The expression of detoxification genes in two maize cultivars by interaction of isoxadifen-ethyl and nicosulfuron

Abstract

Herbicide safeners protect crop plants from herbicide phytotoxicity, but an understanding of their molecular mechanisms is still lacking. We investigated the effects of the safener isoxadifen-ethyl and/or nicosulfuron on the expression of 10 genes, 8 glutathione transferases (GSTs), 1 glutathione transporter and 1 multidrug resistance protein gene in two maize cultivars. Nicosulfuron and isoxadifen-ethyl induce different detoxification enzyme genes. The expression analyses of the 10 genes revealed that most were expressed much higher in 'Zhengdan958' than those in 'Zhenghuangnuo No.2', both in control and in isoxadifen-ethyl- and/or nicosulfuron-treated plants. The expression levels of ZmGSTIV, ZmGST6, ZmGST31 and ZmMRP1 in two maize cultivars were up-regulated by isoxadifen-ethyl only, or in combination with nicosulfuron, whereas nicosulfuron down-regulated the expression of eight genes. Thus, ZmGSTIV, ZmGST6, ZmGST31 and ZmMRP1 could be considered safener-responsive and may be the core genes responsible for isoxadifen-ethyl increasing the tolerance of maize to nicosulfuron.

Keywords: Detoxification gene expression; Herbicide phytotoxicity; Isoxadifen-ethyl; Maize.