Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA

Methods Mol Biol. 2018:1802:101-113. doi: 10.1007/978-1-4939-8546-3_7.

Abstract

Next-generation sequencing (NGS) is increasingly recognized for its ability to deliver high-resolution and high-throughput HLA genotyping. As a result, there is active interest in applying NGS technologies to perform high volume bone marrow donor recruitment typing. Currently, buccal-based DNA specimens are considered a noninvasive and cost-effective method for registry typing. Here, we describe the feasibility of using long-range PCR and clonal sequencing by Illumina MiSeq to deliver unambiguous HLA typing on buccal-based donor recruitment samples.

Keywords: Buccal extracted DNA; Donor recruitment samples; Human leukocyte antigen; Illumina MiSeq; Next-generation sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / genetics*
  • DNA / isolation & purification*
  • Data Analysis
  • Gene Library
  • HLA Antigens / genetics
  • High-Throughput Nucleotide Sequencing / methods*
  • Histocompatibility Testing / methods*
  • Humans
  • Mouth / metabolism*

Substances

  • HLA Antigens
  • DNA