Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules

You Wang; David S Letham; Peter C L John; Ren Zhang

doi:10.1007/978-1-4939-7871-7_14

Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules

Methods Mol Biol. 2018:1794:225-234. doi: 10.1007/978-1-4939-7871-7_14.

Authors

You Wang¹, David S Letham², Peter C L John², Ren Zhang³

Affiliations

¹ School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia.
² Research School of Biology, Australian National University, Canberra, ACT, Australia.
³ School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia. rzhang@uow.edu.au.

PMID: 29855960
DOI: 10.1007/978-1-4939-7871-7_14

Abstract

Protein-small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.

Keywords: Protein–small molecule interactions; Yeast three-hybrid system; Yeast two-hybrid system.

MeSH terms

Protein Binding
Proteins / metabolism*
Saccharomyces cerevisiae / metabolism*
Small Molecule Libraries / metabolism*
Two-Hybrid System Techniques*

Substances

Proteins
Small Molecule Libraries