Reclassification of Borrelia spp. Isolated in South Korea Using Multilocus Sequence Typing

Jpn J Infect Dis. 2018 Sep 21;71(5):350-353. doi: 10.7883/yoken.JJID.2018.139. Epub 2018 May 31.

Abstract

Here, we used multilocus sequence typing (MLST) to evaluate 3 intergenic genes (16S rRNA, ospA, and 5S-23S IGS) in Borrelia isolated from South Korea to analyze the relationships between host, vector, and molecular background. We identified B. afzelii, B. yangtzensis, B. garinii, and B. bavariensis. This study is the first report for the identification of B. yangtzensis using MLST in South Korea.

Keywords: 16S rRNA; 5S-23S IGS; Borrelia; MLST; ospA.

MeSH terms

  • Animals
  • Antigens, Surface / genetics
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Vaccines / genetics
  • Borrelia / classification*
  • Borrelia / genetics
  • Borrelia / isolation & purification*
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Genotype*
  • Ixodes / microbiology
  • Lipoproteins / genetics
  • Multilocus Sequence Typing*
  • Murinae / microbiology
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea

Substances

  • Antigens, Surface
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • DNA, Bacterial
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer
  • Lipoproteins
  • OspA protein
  • RNA, Ribosomal, 16S