Objectives: Although different methods have been established to detect epidermal growth factor receptor (EGFR) T790M mutation in circulating tumor DNA (ctDNA), a wide range of diagnostic accuracy values were reported in previous studies. The aim of this meta-analysis was to provide pooled diagnostic accuracy measures for droplet digital PCR (ddPCR) in the diagnosis of EGFR T790M mutation based on ctDNA.
Materials and methods: A systematic review and meta-analysis were carried out based on resources from Pubmed, Web of Science, Embase and Cochrane Library up to October 11, 2017. Data were extracted to assess the pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio (NLR), diagnostic OR (DOR), and areas under the summary receiver-operating characteristic curve (SROC).
Results: Eleven of 311 studies identified have met the including criteria. The sensitivity and specificity of ddPCR for the detection of T790M mutation in ctDNA ranged from 0.0% to 100.0% and 63.2% to 100.0%, respectively. For the pooled analysis, ddPCR had a performance of 70.1% (95% CI, 62.7%-76.7%) sensitivity, 86.9 % (95% CI, 80.6%-91.7%) specificity, 3.67 (95% CI, 2.33-5.79) PLR, 0.41 (95% CI, 0.32-0.55) NLR, and 10.83 (95% CI, 5.86-20.03) DOR, with the area under the SROC curve being 0.82.
Conclusion: The ddPCR harbored a good performance for detection of EGFR T790M mutation in ctDNA.
Keywords: T790M; circulating tumor DNA; droplet digital PCR; lung cancer.