Thiol-ene click chemistry towards easy microarraying of half-antibodies

Chem Commun (Camb). 2018 Jun 12;54(48):6144-6147. doi: 10.1039/c8cc01369a.

Abstract

A UV light-induced thiol-ene coupling reaction (TEC) between half-antibodies (hIgG) and vinyl functionalized glass surfaces was run for biosensing in the microarray format. The accomplished performance improved that obtained with whole antibodies.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology
  • Biosensing Techniques / methods
  • C-Reactive Protein / analysis
  • C-Reactive Protein / immunology
  • Carbocyanines / chemistry
  • Cattle
  • Click Chemistry / methods
  • Fluorescent Dyes / chemistry
  • Humans
  • Immunoassay / instrumentation
  • Immunoglobulin G / chemistry*
  • Immunoglobulin G / immunology*
  • Limit of Detection
  • Microarray Analysis / instrumentation
  • Oxidation-Reduction
  • Serum Albumin, Bovine
  • Sulfhydryl Compounds / chemistry*
  • Sulfhydryl Compounds / radiation effects
  • Troponin I / blood
  • Troponin I / immunology
  • Ultraviolet Rays
  • Vinyl Compounds / chemistry*
  • Vinyl Compounds / radiation effects

Substances

  • Alexa Fluor 647
  • Antibodies, Monoclonal
  • Carbocyanines
  • Fluorescent Dyes
  • Immunoglobulin G
  • Sulfhydryl Compounds
  • Troponin I
  • Vinyl Compounds
  • Serum Albumin, Bovine
  • C-Reactive Protein