Exploiting the Versatility of Polydopamine-Coated Nanoparticles to Deliver Nitric Oxide and Combat Bacterial Biofilm

Nik Nik M Adnan; Zahra Sadrearhami; Ali Bagheri; Thuy-Khanh Nguyen; Edgar H H Wong; Kitty K K Ho; May Lim; Naresh Kumar; Cyrille Boyer

doi:10.1002/marc.201800159

Exploiting the Versatility of Polydopamine-Coated Nanoparticles to Deliver Nitric Oxide and Combat Bacterial Biofilm

Macromol Rapid Commun. 2018 Jul;39(13):e1800159. doi: 10.1002/marc.201800159. Epub 2018 May 28.

Authors

Nik Nik M Adnan^{1

2}, Zahra Sadrearhami^{1

2}, Ali Bagheri^{1

2

3}, Thuy-Khanh Nguyen^{1

2}, Edgar H H Wong^{1

2}, Kitty K K Ho⁴, May Lim³, Naresh Kumar⁴, Cyrille Boyer^{1

2}

Affiliations

¹ Australian Centre for NanoMedicine (ACN), School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
² Centre for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
³ School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
⁴ School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.

PMID: 29806143
DOI: 10.1002/marc.201800159

Abstract

In this study, an antimicrobial platform in the form of nitric oxide (NO) gas-releasing polydopamine (PDA)-coated iron oxide nanoparticles (IONPs) is developed for combating bacterial biofilms. NO is bound to the PDA-coated IONPs via the reaction between NO and the secondary amine moieties on PDA to form N-diazeniumdiolate (NONOate) functionality. To impart colloidal stability to the nanoparticles in aqueous solutions (e.g., phosphate buffered saline (PBS) and bacteria cell culture media M9), a polymer bearing hydrophilic and amine pendant groups, P(OEGMA)-b-P(ABA), is synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization and is subsequently grafted onto the PDA-coated IONPs by employing the Schiff base/Michael addition reaction between o-quinone and a primary amine. These nanoparticles are able to effectively disperse Pseudomonas aeruginosa biofilms (up to 79% dispersal) at submicromolar NO concentrations. In addition, the nanoparticles demonstrate excellent bactericidal activity toward P. aeruginosa planktonic and biofilm cells (up to 5-log₁₀ reduction).

Keywords: biofilms; dopamine; functional; hybrid organic/inorganic nanoparticles; polymerization.

MeSH terms

Biofilms / drug effects*
Biofilms / growth & development
Coated Materials, Biocompatible* / chemistry
Coated Materials, Biocompatible* / pharmacology
Drug Carriers* / chemistry
Drug Carriers* / pharmacology
Indoles / chemistry*
Nanoparticles / chemistry*
Nitric Oxide* / chemistry
Nitric Oxide* / pharmacology
Polymers / chemistry*
Pseudomonas aeruginosa / physiology*

Substances

Coated Materials, Biocompatible
Drug Carriers
Indoles
Polymers
polydopamine
Nitric Oxide