Physicochemical properties and digestibility of endosperm starches in four indica rice mutants

Xin Zhou; Yining Ying; Binglian Hu; Yuehan Pang; Jinsong Bao

doi:10.1016/j.carbpol.2018.04.070

Physicochemical properties and digestibility of endosperm starches in four indica rice mutants

Carbohydr Polym. 2018 Sep 1:195:1-8. doi: 10.1016/j.carbpol.2018.04.070. Epub 2018 Apr 21.

Authors

Xin Zhou¹, Yining Ying², Binglian Hu³, Yuehan Pang¹, Jinsong Bao⁴

Affiliations

¹ Institute of Nuclear Agricultural Sciences, Key Laboratory of Zhejiang Province and Chinese Ministry of Agriculture, Zhejiang University, Huajiachi Campus, Hangzhou, 310029, China.
² Institute of Nuclear Agricultural Sciences, Key Laboratory of Zhejiang Province and Chinese Ministry of Agriculture, Zhejiang University, Huajiachi Campus, Hangzhou, 310029, China; Department of Applied Bioscience, College of Agriculture and Biotechnology, Zhejiang University, Zijinggang Campus, Hangzhou, 310058, China.
³ Department of Applied Bioscience, College of Agriculture and Biotechnology, Zhejiang University, Zijinggang Campus, Hangzhou, 310058, China.
⁴ Institute of Nuclear Agricultural Sciences, Key Laboratory of Zhejiang Province and Chinese Ministry of Agriculture, Zhejiang University, Huajiachi Campus, Hangzhou, 310029, China; Department of Applied Bioscience, College of Agriculture and Biotechnology, Zhejiang University, Zijinggang Campus, Hangzhou, 310058, China. Electronic address: jsbao@zju.edu.cn.

PMID: 29804956
DOI: 10.1016/j.carbpol.2018.04.070

Abstract

Structural, physicochemical characteristics and digestibility of endosperm starches from four mutants (GM01, 03-05) and their parent Guangluai 4 (GLA4) were characterized. GM05 had a little higher apparent amylose content (AAC) and showed little difference in starch and digestion properties from GLA4. However, GM03 and GM04 with increased amount of chalkiness and partial translucent region in the endosperm displayed a distinct starch and digestion properties, which had the RS of 7.9 and 8.4%, respectively. GM03 and GM04 had higher AAC (33-35%), lower degree of crystallinity, lower gelatinization temperature and pasting viscosities, and more amount of B1 (DP 12-24) and B2 (DP 25-36) chains and less amount of B3 chains (DP ≥ 37) in amylopectin. AAC and the amount of B1 chains had positive correlation with RS, but the amount of B3 chains had negative correlation with RS. The results of this study may be applied to design RS by selecting rice germplasm with high AAC and high amount of B1 chains (DP 12-24) of amylopectin.

Keywords: Chain length distribution; Pasting viscosity; Resistant starch; Rice; Starch mutant; Thermal properties.

MeSH terms

Amylopectin / chemistry*
Amylose / chemistry*
Endosperm / chemistry*
Hydrolysis
Mutation*
Oryza / chemistry*
Oryza / genetics
Viscosity

Substances

Amylose
Amylopectin