Fabrication of lecithin-gum tragacanth muco-adhesive hybrid nano-carrier system for in-vivo performance of Amphotericin B

Tooba Jabri; Muhammad Imran; Shafiullah; Komal Rao; Imdad Ali; Muhammad Arfan; Muhammad Raza Shah

doi:10.1016/j.carbpol.2018.04.013

Fabrication of lecithin-gum tragacanth muco-adhesive hybrid nano-carrier system for in-vivo performance of Amphotericin B

Carbohydr Polym. 2018 Aug 15:194:89-96. doi: 10.1016/j.carbpol.2018.04.013. Epub 2018 Apr 5.

Authors

Tooba Jabri¹, Muhammad Imran², Shafiullah³, Komal Rao⁴, Imdad Ali⁵, Muhammad Arfan⁶, Muhammad Raza Shah⁷

Affiliations

¹ International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. Electronic address: toobaasif137@gmail.com.
² International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. Electronic address: imranbjr.khan@gmail.com.
³ International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. Electronic address: shafi_ullah34@yahoo.com.
⁴ International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. Electronic address: komalrao665@yahoo.com.
⁵ International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. Electronic address: imdadchem26@gmail.com.
⁶ International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. Electronic address: m_arfan@upesh.edu.pk.
⁷ International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. Electronic address: raza.shah@iccs.edu.

PMID: 29801862
DOI: 10.1016/j.carbpol.2018.04.013

Abstract

Nano-carriers are excellent systems for improving bioavailability of poor aqueous soluble drugs. This study focuses fabrication of lecithin-gum tragacanth muco-adhesive hybrid NPs for enhancing Amphotericin B (AmpB) oral bioavailability. AmpB loaded lecithin NPs were synthesized through solvent diffusion method. Green synthesis of stable muco-adhesive gum tragacanth (GT) gold NPs was confirmed through UV-vis spectrophotometer and FT-IR. AmpB loaded lecithin NPs hybrid with GT gold NPs were characterized for shape, size, polydispersity index (PDI), zeta potential, drug entrapment efficiency and drug-excepients interactions using atomic force microscope (AFM), zetasizer, UV-vis spectrophotometer and FT-IR respectively. In-vivo bioavailability of AmpB loaded in NPs was investigated in rabbits. AmpB loaded muco-adhesive NPs were found polydispersed with 358.3 ± 1.78 nm mean size and -19.9 ± 0.51 mV zeta potential. They entrapped 78.91 ± 2.44% AmpB and enhanced its oral bioavailability in animals. Results reveal the hybrid NPs as efficient carriers for enhancing AmpB oral bioavailability in controlled manner.

Keywords: Amphotericin B; Bioavailability; Gum tragacanth; Hybrid nanoparticles; Lecithin; Muco-adhesive.

MeSH terms

Adhesives / chemical synthesis
Adhesives / chemistry
Administration, Oral
Amphotericin B / administration & dosage
Amphotericin B / chemistry
Amphotericin B / pharmacokinetics*
Animals
Antiprotozoal Agents / administration & dosage
Antiprotozoal Agents / chemistry
Antiprotozoal Agents / pharmacokinetics*
Biological Availability
Diffusion
Drug Carriers / chemistry
Lecithins / chemistry*
Particle Size
Rabbits
Surface Properties
Tragacanth / chemical synthesis
Tragacanth / chemistry*

Substances

Adhesives
Antiprotozoal Agents
Drug Carriers
Lecithins
Amphotericin B
Tragacanth