A near-infrared fluorescent probe for rapid detection of carbon monoxide in living cells

Liqiang Yan; Ding Nan; Cheng Lin; Yi Wan; Qiang Pan; Zhengjian Qi

doi:10.1016/j.saa.2018.05.059

A near-infrared fluorescent probe for rapid detection of carbon monoxide in living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2018 Sep 5:202:284-289. doi: 10.1016/j.saa.2018.05.059. Epub 2018 May 16.

Authors

Liqiang Yan¹, Ding Nan², Cheng Lin², Yi Wan², Qiang Pan², Zhengjian Qi³

Affiliations

¹ College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China. Electronic address: liqiangyan@glut.edu.cn.
² College of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu 211189, PR China.
³ College of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu 211189, PR China. Electronic address: qizhengjian506@163.com.

PMID: 29800891
DOI: 10.1016/j.saa.2018.05.059

Abstract

A near-infrared (NIR) and colorimetric fluorescent probe system was developed for Carbon Monoxide (CO) via a Pd⁰-mediated Tsuji-Trost reaction. In this probe, phenoxide anion formation (DPCO^-) was acted as the signal unit and an allyl carbonate group was used as the recognition unit. This non-fluorescent probe molecule can release the relevant fluorophore after conversion of Pd²⁺ to Pd⁰ by CO. The probe system including probe 1 and Pd²⁺ can be used for "naked-eye" detection of CO, and exhibited high selectivity to CO over various other sensing objects. More importantly, the probe system has great potential for fluorescence imaging of intracellular CO in living cells.

Keywords: Carbon monoxide; Fluorescence probe; Living cells; Near-infrared.

MeSH terms

Carbon Monoxide / analysis*
Cell Death
Cell Survival
Colorimetry
Fluorescent Dyes / chemistry*
Humans
MCF-7 Cells
Spectrometry, Fluorescence
Spectroscopy, Near-Infrared*

Substances

Fluorescent Dyes
Carbon Monoxide