A novel bioassay based on aptamer-functionalized magnetic nanoparticle for the detection of zearalenone using time resolved-fluorescence NaYF4: Ce/Tb nanoparticles as signal probe

Sobia Niazi; Xiaole Wang; Imran Pasha; Imran Mahmood Khan; Sen Zhao; Muhammad Shoaib; Shijia Wu; Zhouping Wang

doi:10.1016/j.talanta.2018.04.013

A novel bioassay based on aptamer-functionalized magnetic nanoparticle for the detection of zearalenone using time resolved-fluorescence NaYF₄: Ce/Tb nanoparticles as signal probe

Talanta. 2018 Aug 15:186:97-103. doi: 10.1016/j.talanta.2018.04.013. Epub 2018 Apr 13.

Authors

Sobia Niazi¹, Xiaole Wang², Imran Pasha³, Imran Mahmood Khan², Sen Zhao⁴, Muhammad Shoaib², Shijia Wu⁵, Zhouping Wang⁶

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China.
³ National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China.
⁵ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi, China.
⁶ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi, China. Electronic address: wangzp@jiangnan.edu.cn.

PMID: 29784425
DOI: 10.1016/j.talanta.2018.04.013

Abstract

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by fungi on stored grains. The earlier detection methods used for ZEN rely on expensive equipment, time-consuming sample preparation and temperature sensitive antibodies. The current work, proposed a novel strategy based on ZEN aptamer labeled with amine-functionalized magnetic nanoparticle (MNPs) as a capture probe and time-resolved fluorescence (TRFL) nanoparticles labeled with complementary DNA (cDNA) as a signal probe. Under the optimized conditions, TRFL intensity at 544 nm was used to measure ZEN (R² = 0.9920) in the range of 0.001-10 ng mL^-1 and limits of detection (LOD) for proposed method was 0.21 pg mL^-1. The specificity of bioassay was also determined by using other mycotoxins (OTA, AFB₂, DON and Patulin) and results showed that the aptamer are specific to recognize only ZEN. The analytical applications of the present bioassay in maize and wheat samples were also examined and results were compared with existing methods. Based on these findings, it is suggested to use current rapid and simple bioassay for the determination of ZEN in food and agricultural products.

Keywords: Aptamer; Bioassay; Magnetic nanoparticles; Time-resolved fluorescence nanoparticles; Zearalenone.

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques*
Cerium / chemistry
Fluorescence
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Fluorides / chemistry
Food Contamination / analysis*
Magnetite Nanoparticles / chemistry*
Nanoparticles / chemistry
Particle Size
Surface Properties
Terbium / chemistry
Time Factors
Triticum / chemistry
Yttrium / chemistry
Zea mays / chemistry
Zearalenone / analysis*

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Magnetite Nanoparticles
sodium yttriumtetrafluoride
Terbium
Cerium
Yttrium
Zearalenone
Fluorides