Bone Area Histomorphometry

J Forensic Sci. 2019 Mar;64(2):486-493. doi: 10.1111/1556-4029.13815. Epub 2018 May 21.

Abstract

Quantifying the amount of cortical bone loss is one variable used in histological methods of adult age estimation. Measurements of cortical area tend to be subjective and additional information regarding bone loss is not captured considering cancellous bone is disregarded. We describe whether measuring bone area (cancellous + cortical area) rather than cortical area may improve histological age estimation for the sixth rib. Mid-shaft rib cross-sections (n = 114) with a skewed sex distribution were analyzed. Ages range from 16 to 87 years. Variables included: total cross-sectional area, cortical area, bone area, relative bone area, relative cortical area, and endosteal area. Males have larger mean total cross-sectional area, bone area, and cortical area than females. Females display a larger mean endosteal area and greater mean relative measure values. Relative bone area significantly correlates with age. The relative bone area variable will provide researchers with a less subjective and more accurate measure than cortical area.

Keywords: age-at-death estimation; bone area; cancellous bone; cortical bone; forensic science; histomorphometry.

MeSH terms

  • Adolescent
  • Adult
  • Age Determination by Skeleton / methods*
  • Aged
  • Aged, 80 and over
  • Cancellous Bone / anatomy & histology*
  • Cortical Bone / anatomy & histology*
  • Female
  • Forensic Anthropology
  • Humans
  • Image Processing, Computer-Assisted
  • Least-Squares Analysis
  • Male
  • Middle Aged
  • Ribs / anatomy & histology*
  • Sex Characteristics
  • Young Adult