Synthesis of bioactive compounds from vermicast isolated actinomycetes species and its antimicrobial activity against human pathogenic bacteria

Microb Pathog. 2018 Aug:121:155-165. doi: 10.1016/j.micpath.2018.05.027. Epub 2018 May 17.

Abstract

The present study was aimed to evaluate the antimicrobial activities of bioactive compounds synthesized from vermicast isolated actinomycetes species. Specifically, the synthesized bioactive compounds were evaluated for their antimicrobial activity against selected Gram + ve and Gram - ve human pathogenic bacteria including Staphylococcus aureus, Bacillus circulans, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli. Interestingly, a total of five different actinomycetes species were recovered from vermicasts. More interestingly, among these potential actinomycetes species, the bioactive compounds synthesized by isolate AS9 showed a significant antibacterial activity and its mean zone of inhibition was found at 11.3 ± 1.6 mm, 9.5 ± 0.91 mm, and 9.9 ± 1.71mm against S. aureus, B. subtilis, and B. circulans, respectively. Furthermore, according to antibacterial activity and spectrum broadness, three of the actinomycetes strains were selected and characterized by conventional methods. Subsequently, the bioactive compound profiling of these isolated actinomycetes strains performed through GC-MS analysis indicating the presence of the bioactive compounds including 3, octadecene (E), behnic alcohol phenol, 2,4-bis(1,1-dimethyl ethyl) 1-nonadecene, 1-heneicosanol, milbemycin 3-eicosene (E), and 1-docosanol.

Keywords: Actinomycetes; Antimicrobial activity; Bioactive compounds; Chemical analysis; Human pathogens; Vermicasts.

MeSH terms

  • Actinobacteria / classification
  • Actinobacteria / isolation & purification
  • Actinobacteria / metabolism*
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Bacillus / drug effects
  • Bacillus subtilis / drug effects
  • DNA, Bacterial / genetics
  • Escherichia coli / drug effects
  • Escherichia coli / metabolism
  • Gas Chromatography-Mass Spectrometry
  • India
  • Phylogeny
  • Pseudomonas aeruginosa / drug effects
  • RNA, Ribosomal, 16S / genetics
  • Spectroscopy, Fourier Transform Infrared
  • Staphylococcus aureus / drug effects

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • RNA, Ribosomal, 16S