Development of human-derived hemoglobin-albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification

Seiichi Ohta; Kenichiro Hashimoto; Xiaoting Fu; Masamichi Kamihira; Yasuyuki Sakai; Taichi Ito

doi:10.1016/j.jbiosc.2018.04.017

Development of human-derived hemoglobin-albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification

J Biosci Bioeng. 2018 Oct;126(4):533-539. doi: 10.1016/j.jbiosc.2018.04.017. Epub 2018 Jun 12.

Authors

Seiichi Ohta¹, Kenichiro Hashimoto², Xiaoting Fu³, Masamichi Kamihira⁴, Yasuyuki Sakai⁵, Taichi Ito⁶

Affiliations

¹ Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
² Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
³ Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
⁴ Department of Chemical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
⁵ Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
⁶ Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. Electronic address: taichi@m.u-tokyo.ac.jp.

PMID: 29776730
DOI: 10.1016/j.jbiosc.2018.04.017

Abstract

Novel hemoglobin-based artificial oxygen carriers are necessary in tissue engineering. We developed human hemoglobin (hHb) and albumin (HSA)-based microspheres using Shirasu porous glass (SPG) membrane emulsification. The obtained microspheres had a uniform size with an average diameter of 15.1 μm measured by optical microscope, which is similar to the diameter of human red blood cells (7-8 μm). The loading amount of hHb in the microspheres was 20 wt%, which is similar to that of red blood cells (33 wt%). The hHb-HSA microspheres showed similar oxygen dissociation behavior and methemoglobin formation resistance to native hHb. Incubation with genetically engineered HeLa cells that shows hypoxia-responsive EGFP expression demonstrated efficient oxygen supply from the microspheres. Our study suggests the utility of hHb-HSA microspheres as oxygen carriers for tissue engineering with a low risk of infectious disease.

Keywords: Hemoglobin; Hypoxia-responsive cell; Microsphere; Oxygen carrier; Shirasu porous glass membrane emulsification; Tissue engineering.

MeSH terms

Albumins / chemistry
Albumins / metabolism*
Emulsions / chemistry
Emulsions / metabolism
Erythrocytes / chemistry
Erythrocytes / metabolism
Glass / chemistry
HeLa Cells
Hemoglobins / chemistry
Hemoglobins / metabolism*
Humans
Membranes
Membranes, Artificial
Microspheres
Oxygen / metabolism*
Particle Size
Porosity

Substances

Albumins
Emulsions
Hemoglobins
Membranes, Artificial
Oxygen