Tumor-dependent secretion of close homolog of L1 results in elevation of its circulating level in mouse model for human lung tumor

Norihiro Kotani; Yui Ida; Takanari Nakano; Izumi Sato; Ryusuke Kuwahara; Arisa Yamaguchi; Masahiro Tomita; Koichi Honke; Takayuki Murakoshi

doi:10.1016/j.bbrc.2018.05.096

Tumor-dependent secretion of close homolog of L1 results in elevation of its circulating level in mouse model for human lung tumor

Biochem Biophys Res Commun. 2018 Jul 2;501(4):982-987. doi: 10.1016/j.bbrc.2018.05.096. Epub 2018 May 22.

Authors

Norihiro Kotani¹, Yui Ida², Takanari Nakano², Izumi Sato², Ryusuke Kuwahara³, Arisa Yamaguchi⁴, Masahiro Tomita⁵, Koichi Honke⁴, Takayuki Murakoshi²

Affiliations

¹ Department of Biochemistry, Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama, 350-0495, Japan. Electronic address: kotani@saitama-med.ac.jp.
² Department of Biochemistry, Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama, 350-0495, Japan.
³ Quantum Wave Microscopy Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-son, Kunigami-gun, Okinawa, 904-0495, Japan.
⁴ Department of Biochemistry, Kochi University Medical School, Nankoku, Kochi, 783-8505, Japan.
⁵ Division of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurima-machiya-cho, Tsu, Mie, 514-8507, Japan.

PMID: 29775614
DOI: 10.1016/j.bbrc.2018.05.096

Abstract

Close homolog of L1 (CHL1) and its truncated form mainly play crucial roles in mouse brain development and neural functions. Herein, we newly identified that truncated form of CHL1 is produced and released from lung tumor tissue in a mouse model expressing human EML4-ALK fusion gene. Both western blot and direct ELISA analysis revealed that mouse CHL1 level in serum (including serum extracellular vesicles) was significantly elevated in EML4-ALK transgenic mice. The correlation between the tumor size and the amount of CHL1 secretion could be examined in this study, and showed a significant positive correlation in a tumor size-dependent manner. Considering these results, the measurement of circulating CHL1 level may contribute to assess a tumor progression in human lung tumor patients.

Keywords: Adhesion molecule; CHL1; EML4-ALK gene; Lung tumor; Tumor marker.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Adhesion Molecules / blood*
Cell Adhesion Molecules / metabolism*
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
HEK293 Cells
Humans
Lung Neoplasms / blood*
Lung Neoplasms / pathology
Mice, Inbred C57BL
Tumor Burden

Substances

Cell Adhesion Molecules
Chl1 protein, mouse