Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions

Jing-Kun Yan; Zhi-Chao Ding; Xianli Gao; Yao-Yao Wang; Yan Yang; Di Wu; He-Nan Zhang

doi:10.1016/j.carbpol.2018.04.019

Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions

Carbohydr Polym. 2018 Aug 1:193:373-382. doi: 10.1016/j.carbpol.2018.04.019. Epub 2018 Apr 5.

Authors

Jing-Kun Yan¹, Zhi-Chao Ding², Xianli Gao², Yao-Yao Wang², Yan Yang³, Di Wu³, He-Nan Zhang⁴

Affiliations

¹ School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China. Electronic address: jkyan_27@163.com.
² School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
³ National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, China; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
⁴ National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, China; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China. Electronic address: henanhaoyun@126.com.

PMID: 29773393
DOI: 10.1016/j.carbpol.2018.04.019

Abstract

In this study, hot water, 0.9% NaCl, citric acid, and 1.25 M NaOH/0.05% NaBH₄ were separately used for the extraction of water-soluble H. erinaceus polysaccharides (HEPs; HEP-W, HEP-S, HEP-C, and HEP-A) from the fruit body of Hericium erinaceus. The physicochemical properties and biological activities were then investigated and compared. Results showed that the extraction solvents exhibited significant effects on the extraction yields, molecular weights, monosaccharide compositions, preliminary structural characteristics, microstructures of HEPs and on their contents, such as neutral sugar, uronic acid, protein, and β-(1 → 3)-glucan. In vitro antioxidant activity assays indicated that HEP-C extracted with citric acid solution showed stronger scavenging abilities on hydroxyl and DPPH radicals and antioxidant capacities than HEP-W and HEP-S. Moreover, HEP-C exhibited the strongest inhibitory effects on α-glycosidase and α-amylase activities. Therefore, HEP-C extracted with citric acid can be developed as a potential bioactive ingredient for applications in food, medicine, and cosmetics industries.

Keywords: Acetic acid (PubChem CID: 176); Acetic anhydride (PubChem CID: 7918); Chloroform (PubChem CID: 6212); Ethanol (PubChem CID: 702); Hericium erinaceus fruit body; In vitro antioxidant activity; N-butyl alcohol (PubChem CID: 263); Physicochemical properties; Pyridine (PubChem CID: 1049); Sodium borohydride (PubChem CID: 22959485); Sodium chloride (PubChem CID: 5234); Sodium hydroxide (PubChem CID: 14798); Solvent extractions; Trifluoroacetic acid (PubChem CID: 6422); Water-soluble polysaccharides.