Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots

Yu-Ping Fu; Li-Xia Li; Bing-Zhao Zhang; Berit Smestad Paulsen; Zhong-Qiong Yin; Chao Huang; Bin Feng; Xing-Fu Chen; Ren-Rong Jia; Xu Song; Xue-Qin Ni; Bo Jing; Fa-Ming Wu; Yuan-Feng Zou

doi:10.1016/j.carbpol.2018.03.065

Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots

Carbohydr Polym. 2018 Aug 1:193:212-220. doi: 10.1016/j.carbpol.2018.03.065. Epub 2018 Mar 30.

Authors

Yu-Ping Fu¹, Li-Xia Li¹, Bing-Zhao Zhang², Berit Smestad Paulsen³, Zhong-Qiong Yin¹, Chao Huang¹, Bin Feng⁴, Xing-Fu Chen⁵, Ren-Rong Jia¹, Xu Song¹, Xue-Qin Ni⁶, Bo Jing¹, Fa-Ming Wu⁷, Yuan-Feng Zou⁸

Affiliations

¹ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, PR China.
² Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, PR China.
³ Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
⁴ Animal Nutrition Institute, Sichuan Agricultural University, Wenjiang 611130, PR China.
⁵ Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, PR China.
⁶ Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, PR China.
⁷ School of Pharmacy, Zunyi Medical University, Zunyi 563003, PR China.
⁸ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, PR China. Electronic address: yuanfengzou@sicau.edu.cn.

PMID: 29773375
DOI: 10.1016/j.carbpol.2018.03.065

Abstract

The inulin-type fructan was obtained by DEAE anion exchange chromatography from C. pilosula Nannf. var. modesta (Nannf.) L. T. Shen, after optimized extract condition, which was established by response surface methodology, designed using Box-Behnken factorial design and the optimum condition were: extracting 2.5 h with ratio of solvent to material 40 mL/g at 100 °C, twice. The maximum extraction yield was 20.6 ± 0.2%. It was confirmed as β-(2-1) linkage fructan, with terminal glucose, and with a degree of polymerization of 2-17 (DP_av = 6), shown by the results of methanolysis, methylation, nuclear magnetic resonance and molecular weight determination. The prebiotic activity was proven on account of stimulation effect on Lactobacillus and pH reduction of medium in vitro. The results indicated that the inulin from C. pilosula could be used as a potential natural source of prebiotics.

Keywords: Codonopsis pilosula; Inulin-type fructan; Optimization; Prebiotic activity.

MeSH terms

Chromatography, Ion Exchange
Codonopsis / chemistry*
Fructans / chemistry
Fructans / isolation & purification*
Hydrogen-Ion Concentration
Inulin / chemistry*
Plant Roots / chemistry*
Prebiotics / analysis*

Substances

Fructans
Prebiotics
Inulin