Modification of native fucoidan from Fucus evanescens by recombinant fucoidanase from marine bacteria Formosa algae

Artem S Silchenko; Anton B Rasin; Mikhail I Kusaykin; Olesya S Malyarenko; Natalie M Shevchenko; Anastasya O Zueva; Anatoly I Kalinovsky; Tatyana N Zvyagintseva; Svetlana P Ermakova

doi:10.1016/j.carbpol.2018.03.094

Modification of native fucoidan from Fucus evanescens by recombinant fucoidanase from marine bacteria Formosa algae

Carbohydr Polym. 2018 Aug 1:193:189-195. doi: 10.1016/j.carbpol.2018.03.094. Epub 2018 Mar 30.

Authors

Artem S Silchenko¹, Anton B Rasin², Mikhail I Kusaykin³, Olesya S Malyarenko⁴, Natalie M Shevchenko⁵, Anastasya O Zueva⁶, Anatoly I Kalinovsky⁷, Tatyana N Zvyagintseva⁸, Svetlana P Ermakova⁹

Affiliations

¹ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: artem.silchencko@yandex.ru.
² Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: abrus__54@mail.ru.
³ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: mik@piboc.dvo.ru.
⁴ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: vishchuk@mail.ru.
⁵ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: natalyshe@piboc.dvo.ru.
⁶ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1); Far-Eastern Federal University, Vladivostok, 690022, 8, Sukhanova st., Russia. Electronic address: zstasya95@gmail.com.
⁷ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: kaaniv@pidoc.dvo.ru.
⁸ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: zvyag@piboc.dvo.ru.
⁹ Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1). Electronic address: swetlana_e@mail.ru.

PMID: 29773371
DOI: 10.1016/j.carbpol.2018.03.094

Abstract

Enzymatic depolymerization of fucoidans attracts many researchers due to the opportunity of obtaining standardized fucoidan fragments. Fucoidanase catalyzes the cleavage of fucoidan from Fucus evanescens (FeF) to form low molecular weight products (LMP) and a polymeric fraction (HMP) with 50.8 kDa molecular weight and more than 50% yield. NMR spectroscopy shows that the HMP fraction has regular structure and consists of a repeating fragment [→3)-α-l-Fucp2,4OSO₃^--(1 → 4)-α-l-Fucp2,4OSO₃^--(1 → 4)-α-l-Fucp2OSO₃^--(1→]_n. The anticancer effects of FeF fucoidan and its derivative (HMP) were studied in vitro on colon cancer cells HCT-116, HT-29, and DLD-1. The anticancer activity of the HMP fraction was found to be slightly lower than that of the FeF fucoidan. Research and practical applications of the enzyme include modification of native fucoidans for purposes of regular and easier characterized derivatives acquisition.

Keywords: Anticancer activity; Endo-fucoidanase; Fucoidan depolymerization; Fucoidan from Fucus evanescens; Fucoidanase; Marine bacteria.

MeSH terms

Antineoplastic Agents / chemistry
Antineoplastic Agents / metabolism
Antineoplastic Agents / pharmacology*
Biocatalysis
Carbohydrate Conformation
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Fucus / chemistry*
Fucus / enzymology*
Fucus / metabolism
Humans
Hydrolases / chemistry
Hydrolases / metabolism*
Polysaccharides / chemistry
Polysaccharides / metabolism
Polysaccharides / pharmacology*
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Structure-Activity Relationship
Tumor Cells, Cultured

Substances

Antineoplastic Agents
Polysaccharides
Recombinant Proteins
fucoidan
Hydrolases