A simple and rapid LC-MS/MS method for the determination of amphenicols in Nile tilapia

Food Chem. 2018 Oct 1:262:235-241. doi: 10.1016/j.foodchem.2018.04.087. Epub 2018 Apr 24.

Abstract

A quantitative method for analysis of amphenicols (chloramphenicol - CAP, thiamphenicol - TAP and florfenicol - FF) in Nile tilapia using LC-MS/MS is described. A simple sample preparation procedure was optimized using a Plackett-Burman design. The method was validated in accordance with Decision 2002/657/EC. Repeatability and reproducibility were less than 10.7% and 16%, respectively, for all compounds. Recoveries varied from 79.8% to 92.0%. CCα was 0.019, 54.81 and 54.93 μg.kg-1 for CAP, FF and TAP, respectively. CCβ was 0.068, 64.88 and 58.91 μg.kg-1 for CAP, FF and TAP, respectively. Limits of quantification (LOQ) were 12.5 μg.kg-1 for FF and TAP and 0.15 μg.kg-1 for CAP. Nile tilapia fillets (n = 32) analyzed did not contain chloramphenicol. Thiamphenicol was detected in one sample (3.1%) and florfenicol was detected in every sample, all of them at concentrations below the maximum residue limit.

Keywords: Chloramphenicol; Florfenicol; LC–MS/MS; Liquid chromatography; Nile tilapia; Quantitative method; Thiamphenicol.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / analysis
  • Chloramphenicol / analysis*
  • Chromatography, Liquid / methods*
  • Cichlids*
  • Fish Products / analysis*
  • Limit of Detection
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*
  • Thiamphenicol / analogs & derivatives*
  • Thiamphenicol / analysis*

Substances

  • Anti-Bacterial Agents
  • Chloramphenicol
  • florfenicol
  • Thiamphenicol