Simultaneous separation and determination of six arsenic species in Shiitake (Lentinus edodes) mushrooms: Method development and applications

Shuangyang Chen; Biao Yuan; Jiajia Xu; Guitang Chen; Qiuhui Hu; Liyan Zhao

doi:10.1016/j.foodchem.2018.04.036

Simultaneous separation and determination of six arsenic species in Shiitake (Lentinus edodes) mushrooms: Method development and applications

Food Chem. 2018 Oct 1:262:134-141. doi: 10.1016/j.foodchem.2018.04.036. Epub 2018 Apr 13.

Authors

Shuangyang Chen¹, Biao Yuan¹, Jiajia Xu¹, Guitang Chen², Qiuhui Hu³, Liyan Zhao⁴

Affiliations

¹ College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
² Department of Food Quality and Safety, China Pharmaceutical University, Nanjing 210009, China.
³ College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China.
⁴ College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: zhlychen@njau.edu.cn.

PMID: 29751900
DOI: 10.1016/j.foodchem.2018.04.036

Abstract

A method for simultaneously separating six arsenic (As) species was established by ultrasound-assisted extraction-anion exchange chromatography coupled with inductively coupled plasma mass spectrometry. Six As species could be well separated within 15 min when 0.3 M acetic acid and 25 mM NH₄H₂PO₄ were used as the extraction solvent and mobile phase, respectively. Under optimized conditions, the limits of detection and recovery of As species and the relative standard deviation were in the ranges of 0.31-0.59 μg/L, 94.30-102.75%, and 1.63-3.72%, respectively. Furthermore, the established method was successfully applied to fresh Shiitake (Lentinus edodes) mushrooms and processed products. The inorganic As contents of all samples ranged from 0.204 to 0.500 mg/kg, half of samples (>0.400 mg/kg) were close to the maximum contaminant level allowed by the Chinese (0.5 mg/kg). The health risk index estimation results indicated that no particular risk of As exposure to Chinese consumers existed in L. edodes.

Keywords: Arsenic speciation; HPLC-ICP-MS; Lentinus edodes; Ultrasound-assisted extraction.

MeSH terms

Arsenic / analysis
Arsenic / isolation & purification*
Chemical Fractionation / methods*
Chromatography, Ion Exchange / methods*
Mass Spectrometry / methods*
Shiitake Mushrooms / chemistry*
Ultrasonic Waves

Substances

Arsenic