Enzymatic biofuel cell-based self-powered biosensing of protein kinase activity and inhibition via thiophosphorylation-mediated interface engineering

Chem Commun (Camb). 2018 May 24;54(43):5438-5441. doi: 10.1039/c8cc02328j.

Abstract

We developed a facile and ultrasensitive enzymatic biofuel cell (EBFC)-based self-powered biosensor of protein kinase A (PKA) activity and inhibition via thiophosphorylation-mediated interface engineering. The detection limit was down to 0.00022 U mL-1 (S/N = 3). In addition, the PKA activities from MCF-7 and A549 cell lysates were analyzed and achieved reliable results.

MeSH terms

  • Bioelectric Energy Sources*
  • Biosensing Techniques*
  • Cell Line, Tumor
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors*
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Humans
  • MCF-7 Cells
  • Particle Size
  • Phosphorylation / drug effects
  • Protein Kinase Inhibitors / chemistry
  • Protein Kinase Inhibitors / pharmacology*
  • Sulfhydryl Compounds / chemistry
  • Sulfhydryl Compounds / metabolism*

Substances

  • Protein Kinase Inhibitors
  • Sulfhydryl Compounds
  • Cyclic AMP-Dependent Protein Kinases