Abstract
We developed a facile and ultrasensitive enzymatic biofuel cell (EBFC)-based self-powered biosensor of protein kinase A (PKA) activity and inhibition via thiophosphorylation-mediated interface engineering. The detection limit was down to 0.00022 U mL-1 (S/N = 3). In addition, the PKA activities from MCF-7 and A549 cell lysates were analyzed and achieved reliable results.
MeSH terms
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Bioelectric Energy Sources*
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Biosensing Techniques*
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Cell Line, Tumor
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Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors*
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Cyclic AMP-Dependent Protein Kinases / metabolism*
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Humans
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MCF-7 Cells
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Particle Size
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Phosphorylation / drug effects
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Protein Kinase Inhibitors / chemistry
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Protein Kinase Inhibitors / pharmacology*
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Sulfhydryl Compounds / chemistry
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Sulfhydryl Compounds / metabolism*
Substances
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Protein Kinase Inhibitors
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Sulfhydryl Compounds
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Cyclic AMP-Dependent Protein Kinases