Structural modification of titanium surface by octacalcium phosphate via Pulsed Laser Deposition and chemical treatment

I V Smirnov; J V Rau; M Fosca; A De Bonis; A Latini; R Teghil; V I Kalita; A Yu Fedotov; S V Gudkov; A E Baranchikov; V S Komlev

doi:10.1016/j.bioactmat.2017.03.002

Structural modification of titanium surface by octacalcium phosphate via Pulsed Laser Deposition and chemical treatment

Bioact Mater. 2017 Mar 22;2(2):101-107. doi: 10.1016/j.bioactmat.2017.03.002. eCollection 2017 Jun.

Authors

I V Smirnov¹, J V Rau², M Fosca², A De Bonis³, A Latini⁴, R Teghil³, V I Kalita¹, A Yu Fedotov¹, S V Gudkov^{5

6

7}, A E Baranchikov⁸, V S Komlev¹

Affiliations

¹ A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninsky Prospect 49, 119334 Moscow, Russia.
² Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche, Via del Fosso del Cavaliere 100, 00133 Rome, Italy.
³ Dipartimento di Scienze, Università della Basilicata, Viale dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
⁴ Università di Roma "La Sapienza", Dipartimento di Chimica, Piazzale Aldo Moro, 5, 00185 Rome, Italy.
⁵ Lobachevsky State University, Gagarin Ave. 23, 603950 Nizhny Novgorod, Russia.
⁶ Prokhorov Institute of General Physics, Russian Academy of Sciences, Vavilova Street, 38, 119991 Moscow, Russia.
⁷ Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street, 3, Pushchino, 142290 Moscow Region, Russia.
⁸ Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Leninsky Prospect 31, 119991 Moscow, Russia.

Abstract

In the present study, the Pulsed Laser Deposition (PLD) technique was applied to coat titanium for orthopaedic and dental implant applications. Calcium carbonate (CC) was used as starting coating material. The deposited CC films were transformed into octacalcium phosphate (OCP) by chemical treatments. The results of X-ray diffraction (XRD), Raman, Fourier Transform Infrared Spectroscopy (FTIR) and scanning electron microscopy (SEM) studies revealed that the final OCP thin films are formed on the titanium surface. Human myofibroblasts from peripheral vessels and the primary bone marrow mesenchymal stromal cells (BMMSs) were cultured on the investigated materials. It was shown that all the investigated samples had no short-term toxic effects on cells. The rate of division of myofibroblast cells growing on the surface and saturated BMMSs concentration for the OCP coating were about two times faster than of cells growing on the CC films.

Keywords: Calcium carbonate; Coating; Octacalcium phosphate; Pulsed Laser Deposition; Titanium implants.