DPPC Bilayers in Solutions of High Sucrose Content

Mattia I Morandi; Mathieu Sommer; Monika Kluzek; Fabrice Thalmann; André P Schroder; Carlos M Marques

doi:10.1016/j.bpj.2018.04.003

DPPC Bilayers in Solutions of High Sucrose Content

Biophys J. 2018 May 8;114(9):2165-2173. doi: 10.1016/j.bpj.2018.04.003.

Authors

Mattia I Morandi¹, Mathieu Sommer¹, Monika Kluzek¹, Fabrice Thalmann¹, André P Schroder¹, Carlos M Marques²

Affiliations

¹ Université de Strasbourg, CNRS, Institut Charles Sadron, UPR022, Strasbourg Cedex, France.
² Université de Strasbourg, CNRS, Institut Charles Sadron, UPR022, Strasbourg Cedex, France. Electronic address: marques@unistra.fr.

Abstract

The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease in the transition enthalpy but only a minor shift in the transition temperature. Our observations can be quantitatively accounted for by a thermodynamic model that assumes partial delayed melting induced by sucrose adsorption at the membrane interface.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

1,2-Dipalmitoylphosphatidylcholine / chemistry*
Lipid Bilayers / chemistry*
Solutions
Sucrose / chemistry*
Thermodynamics
Transition Temperature

Substances

Lipid Bilayers
Solutions
1,2-Dipalmitoylphosphatidylcholine
Sucrose