Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview

Anat Rec (Hoboken). 2018 Oct;301(10):1618-1627. doi: 10.1002/ar.23843. Epub 2018 May 13.

Abstract

Peripheral nerve injury results in profound alterations of the affected neurons resulting from the interplay between intrinsic and extrinsic molecular events. Restarting the neuronal regenerative program is an important prerequisite for functional recovery of the injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia provide a useful in vivo and in vitro model for studying the mechanisms that regulate intrinsic neuronal regeneration capacity following axotomy. These studies frequently need to indicate the regenerative status of the corresponding neurons. We summarize the critical issues regarding immunohistochemical detection of several regeneration-associated proteins as markers for the initiation of the regeneration program in rat primary sensory neurons and indicators of axon regeneration in the peripheral nerves. This overview also includes our own results of GAP43 and SCG10 expression in different DRG neurons following double immunostaining with molecular markers of neuronal subpopulations (NF200, CGRP, and IB4) as well as transcription factors (ATF3 and activated STAT3) following unilateral sciatic nerve injury. Anat Rec, 301:1618-1627, 2018. © 2018 Wiley Periodicals, Inc.

Keywords: degeneration; peripheral nerve; regeneration; therapies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Axons / metabolism*
  • Biomarkers / metabolism*
  • Carrier Proteins / metabolism
  • Cornified Envelope Proline-Rich Proteins / metabolism
  • GAP-43 Protein / metabolism
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / metabolism
  • Membrane Proteins / metabolism
  • Microtubule Proteins
  • Nerve Regeneration*
  • Sensory Receptor Cells / classification
  • Sensory Receptor Cells / physiology*
  • Stress, Physiological

Substances

  • Biomarkers
  • Carrier Proteins
  • Cornified Envelope Proline-Rich Proteins
  • GAP-43 Protein
  • Membrane Proteins
  • Microtubule Proteins
  • Sprr1a protein, mouse
  • Stmn2 protein, rat