Cortical Hemodynamic Responses Under Focused Ultrasound Stimulation Using Real-Time Laser Speckle Contrast Imaging

Yi Yuan; Yanchao Zhao; Hongshuai Jia; Mengyang Liu; Shuo Hu; Yingwei Li; Xiaoli Li

doi:10.3389/fnins.2018.00269

Cortical Hemodynamic Responses Under Focused Ultrasound Stimulation Using Real-Time Laser Speckle Contrast Imaging

Front Neurosci. 2018 Apr 23:12:269. doi: 10.3389/fnins.2018.00269. eCollection 2018.

Authors

Yi Yuan¹, Yanchao Zhao¹, Hongshuai Jia², Mengyang Liu³, Shuo Hu¹, Yingwei Li⁴, Xiaoli Li⁵

Affiliations

¹ Institute of Electrical Engineering, Yanshan University, Qinhuangdao, China.
² School of Clinical Medicine, North China University of Science and Technology, Tangshan, China.
³ Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria.
⁴ Institute of Information Engineering, Yanshan University, Qinhuangdao, China.
⁵ State Key Laboratory of Cognitive Neuroscience and Learning and IDG, McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.

Abstract

Although there is increasing use of focused ultrasound stimulation (FUS) in brain studies, the real-time changes of the cerebral blood flow (CBF) due to FUS remain unclear. In this study, we developed a novel scheme combining FUS and laser speckle contrast imaging, which can be used to measure the CBF caused by FUS in real time. The results showed that the change of CBF increased from 0 to 30 s and reached up to the maximum of 115.1 ± 6.5% at 30 s and then decreased gradually from 30 to 60 s. This study demonstrates that FUS was able to increase CBF and alter cortical hemodynamic responses, which indicates that FUS is a potential non-invasive method to study ischemic stroke rehabilitation.

Keywords: cerebral blood flow; focused ultrasound stimulation; laser speckle contrast imaging; rat; real-time.