The objective of the present study was to examine the adaptation strategy of Amorphophallus xiei, a shade-demanding species, grown under different levels of light intensity. The responses of leaf to photosynthetic active radiation, CO2 and simulated sunflecks were analyzed in A. xiei grown under 100% (high light), 32.6% (moderate light) and 5.98% (low light) of full sun. Meanwhile, chlorophyll a fluorescence parameter and light energy partitioning were also recorded and calculated in the above-mentioned responsive process. The results showed that in most cases, the maximum photosynthetic rate (Pmax), dark respiration rate, apparent quantum yield and carboxylation efficiency in A. xiei significantly decreased with increasing the light level, however, the light compensation point, CO2 compensation point significantly increased. The photosynthetic induction was quicker in individuals grown under moderate light (P<0.05), and the initial stomatal conductance (gs-i) during dark adaptation increased significantly with increasing the light level. There was a ne-gative correlation between gs-i and the time required to reach 30%, 50% and 90% of Pmax during the process of photosynthetic induction. Moreover, the values of actual photochemical efficiency of PS2 (ΔF/Fm') in the light, phototochemical quenching of chlorophyll fluorescence (qP) and photosynthetic electron transport rate (ETR) were higher and non-photochemical quenching (NPQ) recorded in photosynthetic induction was also higher in individuals grown under high light, nevertheless, the maximum photochemical efficiency of PS2 in the light (Fv'/Fm') was higher in individuals grown under low light. The proportion of light energy allocated to non-photochemical quenching (ФNPQ) was lower in individuals grown under high light, and, correspondingly, it was higher in ones grown under low light. The results obtained here suggested that, when exposed to high light stress, moderate- and low-light-grown A. xiei would activate the mechanism of energy dissipation to protect itself from injury. Correspondingly, high-light-grown individuals would employ the strategy of increasing heat dissipation and forming quenching complex to cope with high light stress, which, however, might be one of reasons for the sensitivity of A. xiei to high light environment.
为了探讨喜阴植物谢君魔芋(Amorphophallus xiei)对不同光强的适应策略,测量和分析了不同透光率(高光,透光率100%;中光,透光率32.6%;低光,透光率5.98%)下谢君魔芋对光、CO2、光斑的响应特征及响应过程中叶绿素a荧光和能量分配特征.结果表明: 随着生长光强的增大,谢君魔芋最大净光合速率(Pmax)、暗呼吸速率、表观量子产额、羧化效率显著降低,光补偿点、CO2补偿点显著升高.中光处理的谢君魔芋对光合诱导的响应更迅速(P<0.05);随着生长光强的增加,暗适应初始气孔导度(gs-i)显著升高;完成光合诱导中最大净光合速率30%(t30%P)、50%(t50%P)和90%(t90%P)所需的时间与gs-i呈负相关.高光处理的植株PSⅡ实际光化学效率(ΔF/Fm′)、光化学猝灭(qP)和电子传递速率(ETR)较高,且在光合诱导过程中所对应的非光化学猝灭(NPQ)值相对较高,而低光处理具有较高的反应中心激发能捕获效率(Fv′/Fm′).高光处理非光化学耗散途径比例(ФNPQ)较低,而低光处理ФNPQ则相对较高.表明喜阴植物谢君魔芋在中低光下生长时受到高光胁迫能够启动快速耗散机制来保护自身光合机构,长期处于高光环境则采用增加热耗散成本和形成淬灭复合物的策略在一定程度上应对高光胁迫,这可能是其不能很好适应高光环境的原因之一.
Keywords: Amorphophallus xiei; energy partitioning; fluorescence characteristics; light intensity; photosynthetic characteristics.