Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways

Ta-Kuo Juan; Kuo-Ching Liu; Chao-Lin Kuo; Mei-Due Yang; Yung-Lin Chu; Jiun-Long Yang; Ping-Ping Wu; Yi-Ping Huang; Kuang-Chi Lai; Jing-Gung Chung

doi:10.3892/ol.2018.8286

Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways

Oncol Lett. 2018 May;15(5):7716-7724. doi: 10.3892/ol.2018.8286. Epub 2018 Mar 16.

Authors

Ta-Kuo Juan¹, Kuo-Ching Liu², Chao-Lin Kuo³, Mei-Due Yang⁴, Yung-Lin Chu⁵, Jiun-Long Yang², Ping-Ping Wu⁶, Yi-Ping Huang⁷, Kuang-Chi Lai^{8

9

10}, Jing-Gung Chung^{1

11}

Affiliations

¹ Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan, R.O.C.
² Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan, R.O.C.
³ Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan, R.O.C.
⁴ Department of Surgery, China Medical University Hospital, Taichung 404, Taiwan, R.O.C.
⁵ International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung 912, Taiwan, R.O.C.
⁶ School of Pharmacy, China Medical University, Taichung 404, Taiwan, R.O.C.
⁷ Department of Physiology, China Medical University, Taichung 404, Taiwan, R.O.C.
⁸ School of Medicine, China Medical University, Taichung 404, Taiwan, R.O.C.
⁹ Department of Medical Laboratory Science and Biotechnology, College of Medicine and Life Science, Chung Hwa University of Medical Technology, Tainan 717, Taiwan, R.O.C.
¹⁰ Department of Surgery, China Medical University Beigang Hospital, Beigang, Yunlin 651, Taiwan, R.O.C.
¹¹ Department of Biotechnology, Asia University, Wufeng, Taichung 413, Taiwan, R.O.C.

Abstract

Tetrandrine (TET) exhibits biological activities, including anticancer activity. In Chinese medicine, TET has been used to treat hypertensive and arrhythmic conditions and has been demonstrated to induce cytotoxic effects on human cancer cell lines. However, to the best of the author's knowledge, no previous studies have revealed that TET affects cell metastasis in SW620 human colon cancer cells. The present study demonstrated that TET decreased the cell number and inhibited cell adhesion and mobility of SW620 cells. Furthermore, a wound healing assay was performed to demonstrate that TET suppressed cell movement, and Transwell chamber assays were used to reveal that TET suppressed the cell migration and invasion of SW620 cells. Western blotting demonstrated that TET significantly reduced protein expression levels of SOS Ras/Rac guanine nucleotide exchange factor 1, phosphatidylinositol 3-kinase, growth factor receptor bound protein 2, phosphorylated (p)-c Jun N-terminal kinase 1/2, p-p38, p38, 14-3-3, Rho A, β-catenin, nuclear factor-κB p65, signal transducer and activator of transcription-1 and cyclooxygenase-2, in comparison with untreated SW620 cells. Overall, the results of the present study suggested that TET may be used as a novel anti-metastasis agent for the treatment of human colon cancer in the future.

Keywords: SW620 human colon cancer cells; invasion; matrix metalloproteinase-2; matrix metalloproteinase-9; migration; nuclear factor-κB p65; tetrandrine.