Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing

An Xu; Ruoji Zhou; Jian Tu; Zijun Huo; Dandan Zhu; Donghui Wang; Julian A Gingold; Helen Mata; Pulivarthi H Rao; Mo Liu; Alaa M T Mohamed; Celine Shuet Lin Kong; Brittany E Jewell; Weiya Xia; Ruiying Zhao; Mien-Chie Hung; Dung-Fang Lee

doi:10.1016/j.scr.2018.04.013

Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing

Stem Cell Res. 2018 May:29:215-219. doi: 10.1016/j.scr.2018.04.013. Epub 2018 Apr 27.

Authors

An Xu¹, Ruoji Zhou², Jian Tu³, Zijun Huo⁴, Dandan Zhu¹, Donghui Wang⁵, Julian A Gingold⁶, Helen Mata⁷, Pulivarthi H Rao⁷, Mo Liu¹, Alaa M T Mohamed¹, Celine Shuet Lin Kong¹, Brittany E Jewell², Weiya Xia⁸, Ruiying Zhao¹, Mien-Chie Hung⁹, Dung-Fang Lee¹⁰

Affiliations

¹ Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
² Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA.
³ Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, PR China.
⁴ Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Endocrinology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, PR China.
⁵ Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, PR China.
⁶ Women's Health Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
⁷ Department of Pediatrics, Baylor College of Medicine, Texas Children's Cancer and Hematology Centers, Houston, TX 77030, USA.
⁸ Department of Molecular and Cellular Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
⁹ Department of Molecular and Cellular Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA; Graduate Institute of Biomedical Sciences and Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan.
¹⁰ Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA; Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA. Electronic address: dung-fang.lee@uth.tmc.edu.

Abstract

Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Gene Editing*
Gene Expression Regulation
Homozygote*
Human Embryonic Stem Cells / cytology
Human Embryonic Stem Cells / metabolism*
Humans
Male
Mutation*
Transcription Activator-Like Effector Nucleases*
Tumor Suppressor Protein p53 / genetics*
Tumor Suppressor Protein p53 / metabolism

Substances

TP53 protein, human
Tumor Suppressor Protein p53
Transcription Activator-Like Effector Nucleases

Abstract

Publication types

MeSH terms

Substances

Grants and funding