High flux isothermal assays on the pathogenic features of Mycoplasma pneumoniae

Dingqiang Chen; Donghua Feng; Shuxian Wen; Ling Yang

doi:10.1016/j.micpath.2018.05.004

High flux isothermal assays on the pathogenic features of Mycoplasma pneumoniae

Microb Pathog. 2018 Jul:120:219-222. doi: 10.1016/j.micpath.2018.05.004. Epub 2018 May 4.

Authors

Dingqiang Chen¹, Donghua Feng¹, Shuxian Wen¹, Ling Yang²

Affiliations

¹ Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.
² Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China. Electronic address: jykresearch@126.com.

PMID: 29730516
DOI: 10.1016/j.micpath.2018.05.004

Abstract

As one of the most important pathogens, M. pneumoniae is a causative agent responsible for atypical and other respiratory tract infections, even its extra-pulmonary complications. This study aims to use the high and rapid flux sequencing assays on the M. pneumoniae and further bioinformatic analysis, for the investigation of their clinical features and pathogenic characteristics. The results in this study on the clinical features and pathogenic characteristics of M. pneumoniae may further aid in the control and surveillance and better understanding of this pathogen.

Keywords: High flux assays; Loop mediated isothermal amplification; Mycoplasma pneumoniae; P1 gene.

MeSH terms

Base Sequence
DNA, Bacterial / analysis
Gene Amplification
Genes, Bacterial
Humans
Molecular Diagnostic Techniques / methods*
Mycoplasma pneumoniae / genetics*
Mycoplasma pneumoniae / isolation & purification*
Mycoplasma pneumoniae / pathogenicity
Pneumonia, Mycoplasma / diagnosis*
Respiratory Tract Infections / diagnosis
Respiratory Tract Infections / microbiology
Sensitivity and Specificity

Substances

DNA, Bacterial