Multimodal doxorubicin loaded magnetic nanoparticles for VEGF targeted theranostics of breast cancer

Alevtina S Semkina; Maxim A Abakumov; Alexander S Skorikov; Tatiana O Abakumova; Pavel A Melnikov; Nadejda F Grinenko; Sergey A Cherepanov; Daniil A Vishnevskiy; Victor A Naumenko; Klavdiya P Ionova; Alexander G Majouga; Vladimir P Chekhonin

doi:10.1016/j.nano.2018.04.019

Multimodal doxorubicin loaded magnetic nanoparticles for VEGF targeted theranostics of breast cancer

Nanomedicine. 2018 Jul;14(5):1733-1742. doi: 10.1016/j.nano.2018.04.019. Epub 2018 May 3.

Affiliations

¹ Pirogov Russian National Research Medical University, Moscow, Russian Federation; The National University of Science and Technology MISiS, Moscow, Russian Federation.
² Pirogov Russian National Research Medical University, Moscow, Russian Federation; The National University of Science and Technology MISiS, Moscow, Russian Federation. Electronic address: abakumov1988@gmail.com.
³ Lomonosov Moscow State University, Moscow, Russian Federation.
⁴ Skolkovo Institute of Science and Technology, Moscow, Russian Federation.
⁵ Serbsky National Medical Research Center for Psychiatry and Narcology, Moscow, Russian Federation.
⁶ Pirogov Russian National Research Medical University, Moscow, Russian Federation; Serbsky National Medical Research Center for Psychiatry and Narcology, Moscow, Russian Federation.
⁷ Pirogov Russian National Research Medical University, Moscow, Russian Federation.
⁸ The National University of Science and Technology MISiS, Moscow, Russian Federation.
⁹ The National University of Science and Technology MISiS, Moscow, Russian Federation; Lomonosov Moscow State University, Moscow, Russian Federation; Dmitry Mendeleev University of Chemical Technology of Russia, Moscow, Russian Federation.

PMID: 29730399
DOI: 10.1016/j.nano.2018.04.019

Abstract

In presented paper we have developed new system for cancer theranostics based on vascular endothelial growth factor (VEGF) targeted magnetic nanoparticles. Conjugation of anti-VEGF antibodies with bovine serum albumin coated PEGylated magnetic nanoparticles allows for improved binding with murine breast adenocarcinoma 4T1 cell line and facilitates doxorubicin delivery to tumor cells. It was shown that intravenous injection of doxorubicin loaded VEGF targeted nanoparticles increases median survival rate of mice bearing 4T1 tumors up to 50%. On the other hand magnetic resonance imaging (MRI) of 4T1 tumors 24 h after intravenous injection showed accumulation of nanoparticles in tumors, thus allowing simultaneous cancer therapy and diagnostics.

Keywords: MRI-contrast agents; breast adenocarcinoma; chemotherapy; drug delivery; iron oxide; magnetic nanoparticles.

MeSH terms

Animals
Antibiotics, Antineoplastic / administration & dosage
Antibiotics, Antineoplastic / chemistry
Antibiotics, Antineoplastic / pharmacology*
Doxorubicin / administration & dosage
Doxorubicin / chemistry
Doxorubicin / pharmacology*
Drug Delivery Systems
Female
Magnetite Nanoparticles / administration & dosage*
Magnetite Nanoparticles / chemistry
Mammary Neoplasms, Experimental / drug therapy*
Mammary Neoplasms, Experimental / metabolism
Mammary Neoplasms, Experimental / pathology
Mice
Mice, Inbred BALB C
Multimodal Imaging
Rats
Rats, Wistar
Theranostic Nanomedicine / methods*
Vascular Endothelial Growth Factor A / antagonists & inhibitors*

Substances

Antibiotics, Antineoplastic
Magnetite Nanoparticles
Vascular Endothelial Growth Factor A
vascular endothelial growth factor A, mouse
Doxorubicin