A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi

Yushun Tian; Ting Chen; Peng Luo; Wen Huang; Da Huo; Long Yun; Chaoqun Hu; Chuhang Cheng

doi:10.1016/j.fsi.2018.05.007

A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi

Fish Shellfish Immunol. 2018 Jul:78:364-371. doi: 10.1016/j.fsi.2018.05.007. Epub 2018 May 4.

Authors

Yushun Tian¹, Ting Chen², Peng Luo³, Wen Huang², Da Huo¹, Long Yun¹, Chaoqun Hu², Chuhang Cheng²

Affiliations

¹ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 501301, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
² CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 501301, PR China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, 510301, PR China.
³ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 501301, PR China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, 510301, PR China. Electronic address: luopeng@scsio.ac.cn.

PMID: 29729961
DOI: 10.1016/j.fsi.2018.05.007

Abstract

Fibrinogen-related proteins (FREPs) play a crucial role in invertebrate immune response. In this study, we acquired a novel fibrinogen-related protein gene in Litopenaeus vannamei coding for one kind of fibrinogen-related protein, designated as LvFREP2. The complete cDNA sequence of LvFREP2 was 1903 bp long, containing an open reading frame of 1479 bp coding for LvFREP2. The LvFREP2 protein contained a putative signal peptide and a fibrinogen-related protein domain. qRT-PCRs indicated that LvFREP2 mRNA ubiquitously distributed in all examined tissues, and it was up-regulated in gills after V. harveyi and LPS challenges. The recombinant LvFREP2 agglutinated Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Vibrio alginolyticus, V. cholerae, V. vulnificus, V. parahaemolyticus, V. harveyi, Pseudomonas aeruginosa, P. fluorescens) in a calcium-dependent manner. LvFREP2 also facilitated the clearance of Vibrio harveyi in vivo. Therefore, our results suggested that lvFREP2 may have important roles in the anti-bacterial immunity of L. vannamei.

Keywords: Fibrinogen-related proteins; Innate immunity; Litopenaeus vannamei; Vibrio harveyi.

MeSH terms

Amino Acid Sequence
Animals
Arthropod Proteins / chemistry
Arthropod Proteins / genetics*
Arthropod Proteins / immunology*
Base Sequence
Gene Expression Profiling
Gene Expression Regulation / immunology*
Immunity, Innate / genetics*
Penaeidae / genetics*
Penaeidae / immunology*
Phylogeny

Substances

Arthropod Proteins