Retbindin Is Capable of Protecting Photoreceptors from Flavin-Sensitized Light-Mediated Cell Death In Vitro

Adv Exp Med Biol. 2018:1074:485-490. doi: 10.1007/978-3-319-75402-4_60.

Abstract

Retbindin (Rtbdn) is a novel protein of unknown function found exclusively in the retina. Recently, our group has suggested, from in silico analysis of the peptide sequence and in vitro binding data, that Rtbdn could function to bind riboflavin (RF) and its derivatives flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN), collectively known as flavins. Here we confirm that Rtbdn is capable of flavin binding and that this characteristic can protect photoreceptors from flavin-sensitized light damage.

Keywords: Flavin; Flavoprotein; Photoreceptor; Retbindin; Retina.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Cell Death
  • Cell Line, Tumor
  • Chlorocebus aethiops
  • Culture Media, Conditioned
  • Eye Proteins / pharmacology*
  • Humans
  • Mice
  • Radiation-Sensitizing Agents / pharmacology
  • Recombinant Proteins / pharmacology
  • Retinal Cone Photoreceptor Cells / drug effects*
  • Retinal Cone Photoreceptor Cells / radiation effects
  • Riboflavin / pharmacology

Substances

  • Culture Media, Conditioned
  • Eye Proteins
  • RTBDN protein, human
  • Radiation-Sensitizing Agents
  • Recombinant Proteins
  • Riboflavin