Uptake of BDE-209 on zebrafish embryos as affected by SiO2 nanoparticles

Shu-Ju Chao; C P Huang; Pei-Chung Chen; Shih-Hsien Chang; Chihpin Huang

doi:10.1016/j.chemosphere.2018.04.075

Uptake of BDE-209 on zebrafish embryos as affected by SiO₂ nanoparticles

Chemosphere. 2018 Aug:205:570-578. doi: 10.1016/j.chemosphere.2018.04.075. Epub 2018 Apr 16.

Authors

Shu-Ju Chao¹, C P Huang², Pei-Chung Chen¹, Shih-Hsien Chang³, Chihpin Huang⁴

Affiliations

¹ Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan.
² Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan; Department of Civil and Environmental Engineering, University of Delaware, Newark, De 19716, USA.
³ Department of Public Health, Chung-Shan Medical University, Taichung, 402, Taiwan.
⁴ Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan. Electronic address: cphuang@mail.nctu.edu.tw.

PMID: 29709808
DOI: 10.1016/j.chemosphere.2018.04.075

Abstract

It was hypothesized that interactions between emerging contaminants such as decabromodiphenyl ether (BDE-209) and nanoparticles (NPs) such as nano-SiO₂ (nSiO₂), can affect contaminant transport in the aquatic environment and its ecotoxicity. This study assessed the influence of nSiO₂ on the uptake of BDE-209 by zebrafish embryo. The distribution of BDE-209 and nSiO₂ on the external chorion and the internal embryo mass (i.e., dechorionated embryo) was measured. For single exposure of nSiO₂ to zebrafish embryo, separately, results showed that nSiO₂ accumulation on the chorion surface was higher than that in the dechorionated embryo. The nSiO₂ accumulation on the chorion surface was 129-200 mg-nSiO₂/g-chorion at 48 h post fertilization, hpf, of exposure time, whereas the equilibrium adsorption of nSiO₂ on the dechorionated embryo was ca. 0.42-0.54 mg-nSiO₂/g-embryo at 6 hpf. Results showed that the formation of nSiO₂-BDE-209 associates promoted both extracellular and intracellular uptake of BDE-209 by zebrafish embryo, thereby increasing the bioconcentration of BDE-209 on the chorion surface and in embryo. The results also revealed that the accumulation of BDE-209 on the chorion was remarkably greater than that on the dechorionated embryo at 48 hpf. The uptake of BDE-209 was 17.2 ± 0.45 mg/g-chorion (or 86 ng-BDE-209/chorionated embryo) and 0.37 ± 0.01 mg/g-embryo (or 18.6 ng-BDE-209/dechorionated embryo), respectively, when co-exposure of zebrafish embryos to BDE-209 and nSiO₂. Results from the SEM and EDS analysis revealed that nSiO₂ already passed through the chorion and adhered to the embryo surface/mass.

Keywords: Bioaccumulation; Co-exposure; Decabromodiphenyl ether; Dechorionation; Silica nanoparticles; Zebrafish embryo.

MeSH terms

Adsorption
Animals
Chorion / chemistry
Embryo, Nonmammalian / drug effects
Halogenated Diphenyl Ethers / pharmacokinetics*
Nanoparticles / chemistry*
Silicon Dioxide / pharmacology*
Tissue Distribution
Zebrafish / embryology*

Substances

Halogenated Diphenyl Ethers
Silicon Dioxide
decabromobiphenyl ether