The Association of PPARγ Pro12Ala and C161T Polymorphisms with Polycystic Ovary Syndrome and Their Influence on Lipid and Lipoprotein Profiles

Zohreh Rahimi; Foroogh Chamaie-Nejad; Shohreh Saeidi; Ziba Rahimi; Ali Ebrahimi; Ebrahim Shakiba; Asad Vaisi-Raygani

doi:10.22074/ijfs.2018.5270

The Association of PPARγ Pro12Ala and C161T Polymorphisms with Polycystic Ovary Syndrome and Their Influence on Lipid and Lipoprotein Profiles

Int J Fertil Steril. 2018 Jul;12(2):147-151. doi: 10.22074/ijfs.2018.5270. Epub 2018 Mar 18.

Authors

Zohreh Rahimi^{1

2}, Foroogh Chamaie-Nejad³, Shohreh Saeidi³, Ziba Rahimi¹, Ali Ebrahimi⁴, Ebrahim Shakiba⁵, Asad Vaisi-Raygani^{3

6}

Affiliations

¹ Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
² Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic Address: zrahimi@kums.ac.ir.
³ Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
⁴ Department of Dermatology, Kermanshah University of Medical Sciences, Kermanshah, Iran.
⁵ Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic Address: m.shakiba_d91@yahoo.com.
⁶ Fertlility and Infertility Research Centre, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract

Background: The aim of present study was to clarify the role of the peroxisome proliferator-activated receptor (PPAR) γ Pro12Ala and C161T polymorphisms in the pathogenesis of polycystic ovary syndrome (PCOS) and their influence on lipid and lipoprotein profiles of patients.

Materials and methods: The present cross-sectional study consisted of 50 women with PCOS, who referred to the Kermanshah University of Medical Sciences Clinic between April and October 2015, and 233 unrelated age-matched healthy women from the same region (West Iran). The PPARγ Pro12Ala and PPARγ C161T polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method. Fasting blood sugar (FBS), serum triglycerides (TG), cholesterol, low density lipoprotein- cholesterol (LDL-C), high density lipoproteincholesterol (HDL-C) and estradiol levels were measured.

Results: The serum level of estradiol was significantly lower in PCOS patients compared to healthy women. The PPARγ Pro12Ala (CG) genotype increased the risk of PCOS 2.96-fold. The frequency of the PPARγ T allele (at C161T) was 21% in patients and 17.2% in controls with no significant difference (P=0.52). In all studied individuals, the PPARγ CG genotype was associated with significantly higher levels of TG. However, significantly lower levels of total cholesterol and LDL-C were observed in PPARγ TT individuals compared with those with the CC genotype. Within the PCOS group, the PPARγ CG genotype was significantly associated with lower levels of estradiol compared with the CC genotype. Also, the CG genotype was significantly associated with higher levels of TG when compared with the CC genotype.

Conclusion: Our study shows that, unlike PPARγ C161T, PPARγ Pro12Ala is associated with the risk of PCOS. Also, we found that the lipid and lipoprotein profiles significantly vary based on PPARγ Pro12Ala and C161T genotypes.

Keywords: Estradiol; Lipid; Lipoprotein; Peroxisome Proliferator-Activated Receptor; Polycystic Ovary Syndrome.