The role of nighttime water balance on Olea europaea plants subjected to contrasting water regimes

Abstract

The climate change scenarios besides foreseeing a severe drought imposition also emphasize the temperature rising in the Mediterranean region, with special prominence at nighttime. Despite the high olive tree tolerance to severe environmental conditions, stomatal nighttime water loss can change plant water relations, and the related consequences and opportunities, especially under water scarcity, must be clarified. A set of 3-year-old potted olive trees were subjected to three cycles of drought, imposed by withholding irrigation, while another group were continuously irrigated. At the end of the latter and more severe drought cycle, daytime gas exchange parameters, water status and membrane integrity was negatively affected by drought imposition. Moreover, the nighttime transpiration rate was far above cuticular water loss, suggesting sustained stomatal aperture during nighttime, leading to substantial water losses, which was higher under drought in the first hours of darkness. The higher nighttime stomatal conductance of droughted plants were related with higher starch concentration in their leaves, a thicker trichome layer and a lower intercellular CO₂ concentration, in a closely association with an inferior nighttime respiration. Still, whole-plant transpiration on droughted plants were much lower than leaf transpiration-based estimates, which is interpreted as compensation by water inputs due to dew deposition on leaves. Although unexpected, the increased of stomatal conductance in the first hours of the night, until a certain level of water deficit intensity, could be linked with potential benefits to the plants.

Keywords: Drought; Nocturnal warming; Olive tree; Stomatal opening; Transpiration.