Leaf and canopy reflectance spectrometry applied to the estimation of angular leaf spot disease severity of common bean crops

PLoS One. 2018 Apr 26;13(4):e0196072. doi: 10.1371/journal.pone.0196072. eCollection 2018.

Abstract

This study is aimed at (i) estimating the angular leaf spot (ALS) disease severity in common beans crops in Brazil, caused by the fungus Pseudocercospora griseola, employing leaf and canopy spectral reflectance data, (ii) evaluating the informative spectral regions in the detection, and (iii) comparing the estimation accuracy when the reflectance or the first derivative reflectance (FDR) is employed. Three data sets of useful spectral reflectance measurements in the 440 to 850 nm range were employed; measurements were taken over the leaves and canopy of bean crops with different levels of disease. A system based in Principal Component Analysis (PCA) and Artificial Neural Networks (ANN) was developed to estimate the disease severity from leaf and canopy hyperspectral reflectance spectra. Levels of disease to be taken as true reference were determined from the proportion of the total leaf surface covered by necrotic lesions on RGB images. When estimating ALS disease severity in bean crops by using hyperspectral reflectance spectrometry, this study suggests that (i) successful estimations with coefficients of determination up to 0.87 can be achieved if the spectra is acquired by the spectroradiometer in contact with the leaves, (ii) unsuccessful estimations are obtained when the spectra are acquired by the spectroradiometer from one or more meters above the crop, (iii) the red to near-infrared spectral region (630-850 nm) offers the same precision in the estimation as the blue to near-infrared spectral region (440-850), and (iv) neither significant improvements nor significant detriments are achieved when the input data to the estimation processing system are the FDR spectra, instead of the reflectance spectra.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil
  • Chlorophyll / chemistry
  • Crops, Agricultural
  • Phaseolus / chemistry*
  • Phaseolus / microbiology
  • Plant Diseases / microbiology
  • Plant Leaves / chemistry*
  • Plant Leaves / microbiology
  • Principal Component Analysis
  • Spectrophotometry*

Substances

  • Chlorophyll

Grants and funding

Authors of the article want to thank the financial support provided by FAPEMIG (Minas Gerais State Foundation for Research Development, http://www.fapemig.br) and CNPq Brazilian agencies (http://cnpq.br), that helped to conduct the field experiments of the article. The first author’s work was made possible thanks to a “Formación de Personal Investigador” program grant. This program was financed by the Universidad de Valladolid (www.uva.es), from Spain, and cofinanced by Banco Santander (www.bancosantander.es). Moreover, the collaboration among the authors was possible thanks to a grant program entitled “Becas Iberoamérica. Jóvenes Profesores e Investigadores. Santander Universidades” (https://www.agora-santander.com), financed by Banco Santander. The first and second author were each awarded a grant under this program in 2014, and it financed their stay at the Universidade Federal de Viçosa (MG, Brazil) while the ALS estimation method was implemented and evaluated.