The increasing rate of atmospheric nitrogen (N) deposition has become the focus of research attention. Soil bacterial community plays an important role in soil nutrient cycling. We stimulated N deposition at the Forest Ecosystem of Fujian Normal University and Global Change Research Station in Chenda Town, Sanming City in the Fujian Province of China. We examined the effect of N deposition on the structure and composition of soil bacterial community using 16S rDNA amplification sequencing. The results showed that short-term addition of N had no significant effect on the soil bacterial diversity and composition, but high N treatment significantly affected therelative abundance of individual bacterial species, which increased the abundance of Copiotrophic group and decreased that of the corresponding Oligotrophic group, indicating that changes in soil bacterial nutrient strategies were driven by the availability of nutrients. Enhanced understanding of the responses of soil bacterial community and nutrient distribution pattern to rapid N deposition could improve the prediction ability about the future environment.
大气氮沉降量持续增加已经成为当前关注的热点.土壤细菌群落作为土壤环境中大量存在的微生物,在养分循环过程中发挥着不可忽视的作用.在福建三明森林生态系统与全球变化研究站陈大观测点,我们在野外模拟大气氮沉降试验,通过16S rDNA扩增子测序,研究中亚热带地区杉木幼林土壤细菌群落多样性和组成对氮沉降的响应.结果表明: 短期施氮对研究区的土壤细菌群落多样性和组成并未产生显著影响,但高氮处理显著改变敏感菌群相对丰度,如富营养型类群丰度增加、贫营养型类群丰度降低.土壤细菌群落的营养策略发生变化,这可能是受到了养分有效性的驱动.因此,了解土壤细菌群落和养分分配格局对氮沉降的响应,有助于提高我们对未来环境的预测能力.
Keywords: Lefse analysis; bacterial community; high-throughput sequencing; nitrogen deposition.