Identification of candidate genes mediating apple fruit-cracking resistance following the application of gibberellic acids 4 + 7 and the cytokinin 6-benzyladenine

Abstract

Calyx-end cracking in 'Pink Lady' apple is treated by a solution of gibberellic acids 4 and 7 (GA₄₊₇) and the cytokinin 6-benzyladenine (BA). Although the GA₄₊₇ and BA mixture is applied early in apple fruit development, it mitigates cracking that becomes evident in the mature fruit, implying a long-term treatment effect. The reduced incidence of peel cracking is associated with increased epidermal cell density, which is maintained until fruit maturation. Presently, the expression of genes that have been previously reported to be associated with epidermal cell patterning and cuticle formation, or cracking resistance, was monitored in the peel during fruit development and following GA₄₊₇ and BA treatment. For most of the genes whose expression is naturally upregulated during fruit development, the early GA₄₊₇ and BA treatment maintained or further increased the high expression level in the mature peel. Where the expression of a gene was downregulated during development, no change was detected in the treated mature peel. Gene-networking analysis supported the interaction between gene clusters of cell-wall synthesis, cuticle formation and GA signaling. Overall, the data suggested that the GA₄₊₇ and BA treatment did not modify developmental cues, but promoted or enhanced the innate developmental program.

Keywords: Calyx-end cracking; Cuticle; Epidermal cell density; Malus x domestica; Plant growth regulator.