Mechanical forces have emerged as coordinating signals for most cell functions. Yet, because forces are invisible, mapping tensile stress patterns in tissues remains a major challenge in all kingdoms. Here we take advantage of the adhesion defects in the Arabidopsis mutant quasimodo1 (qua1) to deduce stress patterns in tissues. By reducing the water potential and epidermal tension in planta, we rescued the adhesion defects in qua1, formally associating gaping and tensile stress patterns in the mutant. Using suboptimal water potential conditions, we revealed the relative contributions of shape- and growth-derived stress in prescribing maximal tension directions in aerial tissues. Consistently, the tension patterns deduced from the gaping patterns in qua1 matched the pattern of cortical microtubules, which are thought to align with maximal tension, in wild-type organs. Conversely, loss of epidermis continuity in the qua1 mutant hampered supracellular microtubule alignments, revealing that coordination through tensile stress requires cell-cell adhesion.
Keywords: A. thaliana; cell adhesion; mechanical stress; microtubules; plant biology; plant organs.
The parts of a plant that protrude from the ground are constantly shaken by the wind, applying forces to the plant that it must be able to resist. Indeed, mechanical forces are crucial for the development, growth and life of all organisms and can trigger certain behaviours or the production of particular molecules: for example, forces that bend a plant trigger gene activity that ultimately makes the stem more rigid. Mechanical forces can also originate from inside the organism. For example, the epidermal cells that cover the surface of a plant are placed under tension by the cells in the underlying layers of the plant as they grow and expand. The exact pattern of forces in the plant epidermis was not known because they cannot be directly seen, although scientists have tried to map them using theoretical and computational modeling. A mutant form of the Arabidopsis plant is unable to produce some of the molecules that allow epidermal cells to adhere to each other. Verger et al. placed the mutants in different growth conditions that lowered the pressure inside the plant, and consequently reduced the tension on the epidermal cells. This partly restored the ability of epidermal cells to adhere to each other, although gaps remained between cells in regions of the plant that have been predicted to be under high levels of tension. Verger et al. could therefore use the patterns of the gaps to map the forces across the epidermis, opening the path for the study of the role of these forces in plant development. Further experiments showed that cell adhesion defects prevent the epidermal cells from coordinating how they respond to mechanical forces. There is therefore a feedback loop in the plant epidermis: cell-cell connections transmit tension across the epidermis, and, in turn, tension is perceived by the cells to alter the strength of those connections. The results presented by Verger et al. suggest that plants use tension to monitor the adhesion in the cell layer that forms an interface with the environment. Other organisms may use similar processes; this theory is supported by the fact that sheets of animal cells use proteins that are involved in both cell-cell adhesion and the detection of tension. The next challenge is to analyse how tension in the epidermis affects developmental processes and how a plant responds to its environment.
© 2018, Verger et al.