Novel poly(ε-caprolactone)/amino-functionalized tannin electrospun membranes as scaffolds for tissue engineering

Alessandro F Martins; Suelen P Facchi; Paulo C F da Câmara; Samira E A Camargo; Carlos H R Camargo; Ketul C Popat; Matt J Kipper

doi:10.1016/j.jcis.2018.04.060

Novel poly(ε-caprolactone)/amino-functionalized tannin electrospun membranes as scaffolds for tissue engineering

J Colloid Interface Sci. 2018 Sep 1:525:21-30. doi: 10.1016/j.jcis.2018.04.060. Epub 2018 Apr 16.

Authors

Alessandro F Martins¹, Suelen P Facchi², Paulo C F da Câmara³, Samira E A Camargo³, Carlos H R Camargo³, Ketul C Popat³, Matt J Kipper³

Affiliations

¹ Postgraduate Program in Materials Science & Engineering (PPGCEM), Federal University of Technology (UTFPR-LD), 86036-370 Londrina, PR, Brazil; Postgraduate Program in Environmental Engineering (PPGEA), Federal University of Technology (UTFPR-AP), 86812-460 Apucarana, PR, Brazil; Department of Chemical and Biological Engineering, Colorado State University, 1370 Campus Delivery, Fort Collins, CO, United States. Electronic address: afmartins@utfpr.edu.br.
² Postgraduate Program in Environmental Engineering (PPGEA), Federal University of Technology (UTFPR-AP), 86812-460 Apucarana, PR, Brazil.
³ Department of Chemical and Biological Engineering, Colorado State University, 1370 Campus Delivery, Fort Collins, CO, United States.

PMID: 29680300
DOI: 10.1016/j.jcis.2018.04.060

Abstract

Poly(ε-caprolactone) (PCL) is a hydrophobic and cytocompatible aliphatic polyester that has been used to produce PCL-based nanofibrous for both wound healing and tissue repair. However, the high hydrophobicity and low water adsorptive have been challenges for developing PCL-based materials for use in tissue engineering field. Here, we report a new polymer (a hydrophilic amino-functionalized tannin (TN)) that is associated with PCL for developing PCL-TN blends at different PCL:TN weight ratios (100:0, 95:5, 85:15 and 78:22). PCL:TN ratio may be tuned to modulate hydrophilicity and cytocompatibility of the nanofibers. The neutralization step and surface wettability played an important role in the attachment of human adipose-derived stem cells (ADSC cells) on PCL-TN membranes. Also, fluorescence images confirmed great proliferation of ADSC cells on the PCL-TN electrospun surfaces. Yet, neutralized PCL-TN nanofibers promoted bactericidal activity against Pseudomonas aeruginosa. These membranes have potential to be used as scaffolds for tissue engineering purposes.

Keywords: Cell culture; Electrospinning; Medical application; Membranes; Tannin derivative.