Intranasal delivery of influenza antigen by nanoparticles, but not NKT-cell adjuvant differentially induces the expression of B-cell activation factors in mice and swine

Sankar Renu; Santosh Dhakal; Eunsoo Kim; Jonathan Goodman; Yashavanth S Lakshmanappa; Michael J Wannemuehler; Balaji Narasimhan; Prosper N Boyaka; Gourapura J Renukaradhya

doi:10.1016/j.cellimm.2018.04.005

Intranasal delivery of influenza antigen by nanoparticles, but not NKT-cell adjuvant differentially induces the expression of B-cell activation factors in mice and swine

Cell Immunol. 2018 Jul:329:27-30. doi: 10.1016/j.cellimm.2018.04.005. Epub 2018 Apr 11.

Authors

Sankar Renu¹, Santosh Dhakal¹, Eunsoo Kim², Jonathan Goodman³, Yashavanth S Lakshmanappa¹, Michael J Wannemuehler⁴, Balaji Narasimhan³, Prosper N Boyaka², Gourapura J Renukaradhya⁵

Affiliations

¹ Food Animal Health Research Program, Ohio Agricultural Research and Development Center (OARDC), 1680 Madison Avenue, Wooster, OH 44691, USA; Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
² The Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH 43210, USA.
³ Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA.
⁴ Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011, USA.
⁵ Food Animal Health Research Program, Ohio Agricultural Research and Development Center (OARDC), 1680 Madison Avenue, Wooster, OH 44691, USA; Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA. Electronic address: gourapura.1@osu.edu.

PMID: 29665972
DOI: 10.1016/j.cellimm.2018.04.005

Abstract

Intranasal vaccination of pigs with poly lactic-co-glycolic acid and polyanhydride nanoparticles delivered inactivated influenza virus provides cross-reactive T-cell response, but not antibody response, resulting in incomplete protection and no reduction in nasal virus shedding. Expression of BAFF and Th2 transcription factor GATA-3 were downregulated in lungs of pigs vaccinated with influenza nanovaccine, but in mice it upregulated the expression of BAFF and cytokine TGFβ in cervical lymph nodes. However, the intranasal iNKT cell adjuvant, α-Galctosylceramide upregulates the expression of BAFF in pig lungs. In conclusion, expression of BAFF is differentially regulated by intranasal nanovaccine and α-Galctosylceramide in pig respiratory tract.

Keywords: Antibodies; B-cell activation factors; Mice; Nanoparticles; Pigs; iNKT cells.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Administration, Intranasal / methods
Animals
B-Cell Activating Factor / drug effects*
B-Cell Activating Factor / genetics
Chemotherapy, Adjuvant / methods
Gene Expression Regulation
Influenza Vaccines / administration & dosage
Influenza Vaccines / pharmacology*
Killer Cells, Natural / physiology
Mice
Nanoparticles / therapeutic use
Orthomyxoviridae Infections / prevention & control*
Polyanhydrides / pharmacology
Polylactic Acid-Polyglycolic Acid Copolymer / pharmacology
Swine / immunology
Swine / virology

Substances

B-Cell Activating Factor
Influenza Vaccines
Polyanhydrides
Polylactic Acid-Polyglycolic Acid Copolymer