Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Ilenia Manuguerra; Stefano Croce; Afaf H El-Sagheer; Abhichart Krissanaprasit; Tom Brown; Kurt V Gothelf; Antonio Manetto

doi:10.1039/c8cc00738a

Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Chem Commun (Camb). 2018 May 1;54(36):4529-4532. doi: 10.1039/c8cc00738a.

Authors

Ilenia Manuguerra¹, Stefano Croce, Afaf H El-Sagheer, Abhichart Krissanaprasit, Tom Brown, Kurt V Gothelf, Antonio Manetto

Affiliation

¹ Interdisciplinary Nanoscience Center (iNANO), Gustav Wieds Vej 14 and Department of Chemistry, Aarhus University, Langelandsgade 140, 8000 Aarhus, Denmark.

Abstract

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

MeSH terms

Alkynes / chemistry
Azides / chemistry
Click Chemistry
DNA / chemical synthesis*
DNA / chemistry
DNA / genetics
Green Fluorescent Proteins / genetics*
Mutation
Nanostructures / chemistry*
Oligodeoxyribonucleotides / chemistry*
Polymerase Chain Reaction
Protein Engineering*
Triazoles / chemistry

Substances

Alkynes
Azides
Oligodeoxyribonucleotides
Triazoles
enhanced green fluorescent protein
Green Fluorescent Proteins
DNA