Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa

Yonglong Guo; Qiaolang Zeng; Shiwei Liu; Quan Yu; Peiyuan Wang; Hongjie Ma; Shanshan Shi; Xin Yan; Zekai Cui; Mengyuan Xie; Yunxia Xue; Qingbing Zha; Zhijie Li; Jun Zhang; Shibo Tang; Jiansu Chen

doi:10.1016/j.scr.2018.03.022

Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa

Stem Cell Res. 2018 May:29:139-142. doi: 10.1016/j.scr.2018.03.022. Epub 2018 Apr 6.

Authors

Yonglong Guo¹, Qiaolang Zeng², Shiwei Liu², Quan Yu³, Peiyuan Wang², Hongjie Ma⁴, Shanshan Shi⁵, Xin Yan⁶, Zekai Cui¹, Mengyuan Xie⁷, Yunxia Xue⁸, Qingbing Zha⁵, Zhijie Li⁸, Jun Zhang⁷, Shibo Tang⁹, Jiansu Chen¹⁰

Affiliations

¹ Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China.
² The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China.
³ Centric Laboratory, Medical College, Jinan University, Guangzhou, China.
⁴ Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China.
⁵ Fetal Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, China.
⁶ Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China.
⁷ Key Laboratory of Optoelectronic Information and Sensing Technologies, Guangdong Higher Educational Institutes, Jinan University, Guangzhou 510632, China.
⁸ Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, China; The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China.
⁹ Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China. Electronic address: tangshibo@vip.163.com.
¹⁰ Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, China; The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China; Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China. Electronic address: chenjiansu2000@163.com.

PMID: 29660607
DOI: 10.1016/j.scr.2018.03.022

Abstract

We have established an induced pluripotent stem (iPS) cell line using urine-derived cells from a 27-year-old male patient with retinitis pigmentosa associated with point mutations in the USH2A gene. Feeder-free culture conditions and the integration-free CytoTune™-iPS 2.0 Sendai Reprogramming Kit were used.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Culture Techniques / methods
Cellular Reprogramming Techniques / methods*
Extracellular Matrix Proteins / genetics*
Humans
Induced Pluripotent Stem Cells* / metabolism
Induced Pluripotent Stem Cells* / pathology
Male
Point Mutation*
Retinitis Pigmentosa* / genetics
Retinitis Pigmentosa* / metabolism
Retinitis Pigmentosa* / pathology
Urine*

Substances

Extracellular Matrix Proteins
USH2A protein, human