As an important moral emotion, guilt plays a critical role in social interaction. It has been found that people tended to exhibit prosocial behavior under circumstances of guilt. However, all extant studies have predominantly focused on the influence of guilt on macro-level behavior. So far, no study has investigated whether guilt affects people's micro-level perception. The current study closes this gap by examining whether guilt affects one's inclination to perceive approaching motion. We achieved this aim by probing a facing-the-viewer bias (FTV bias). Specifically, when an ambiguous walking biological motion display is presented to participants via the point-light display technique, participants tend to perceive a walking agent approaching them. We hypothesized that guilt modulated FTV bias. To test this hypothesis, we adopted a two-person situation induction task to induce guilt, whereby participants were induced to feel that because of their poor task performance, their partner did not receive a satisfactory payment. We found that when participants were told that the perceived biological motion was motion-captured from their partner, the FTV bias was significantly increased for guilty participants relative to neutral participants. However, when participants were informed that the perceived biological motion was from a third neutral agent, the FTV bias was not modulated by guilt. These results suggest that guilt influences one's inclination to perceive approaching motion, but this effect is constrained to the person towards whom guilt is directed.