Characterization of αβ and γδ T cell subsets expressing IL-17A in ruminants and swine

Mahmoud M Elnaggar; Gaber S Abdellrazeq; Rohana P Dassanayake; Lindsay M Fry; Victoria Hulubei; William C Davis

doi:10.1016/j.dci.2018.04.003

Characterization of αβ and γδ T cell subsets expressing IL-17A in ruminants and swine

Dev Comp Immunol. 2018 Aug:85:115-124. doi: 10.1016/j.dci.2018.04.003. Epub 2018 Apr 5.

Authors

Mahmoud M Elnaggar¹, Gaber S Abdellrazeq², Rohana P Dassanayake³, Lindsay M Fry⁴, Victoria Hulubei⁵, William C Davis⁵

Affiliations

¹ Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt. Electronic address: mahmoud.elnaggar@wsu.edu.
² Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
³ Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, IA, USA.
⁴ Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA, USA.
⁵ Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA.

PMID: 29627456
DOI: 10.1016/j.dci.2018.04.003

Abstract

As part of our ongoing program to expand immunological reagents available for research in cattle, we developed a monoclonal antibody (mAb) to bovine interleukin-17A (IL-17A), a multifunctional cytokine centrally involved in regulating innate and adaptive immune responses. Initial comparative studies demonstrated the mAb recognizes a conserved epitope expressed on orthologues of IL-17A in sheep, goats and pigs. Comparative flow cytometric analyses of lymphocyte subsets stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin revealed differences in expression of IL-17A by CD4, CD8, and γδ T cells across ruminants and swine species. Results in cattle showed the largest proportion of IL-17A⁺ cells were CD4⁺ followed by γδ and CD8⁺ T cells. Further analysis revealed the IL-17A⁺ γδ T cell subset was comprised of WC1.1⁺, WC1.2⁺, and WC1^- subsets. Analysis of the IL-17A⁺ CD8⁺ T cell subset revealed it was comprised of αβ and γδ T cell subsets. Results in sheep and goats revealed IL-17A is expressed mainly by CD4⁺ and CD8⁺ T cells, with little expression by γδ T cells. Analysis of IL-17A⁺ CD8⁺ T cells showed the majority were CD8⁺ αβ in sheep, whereas they were CD8⁺ γδ in goats. The majority of the sheep and goat IL-17A⁺ γδ T cells were WC1⁺. Results obtained in swine showed expression of IL-17A by CD4, CD8, and γδ T cell subsets were similar to results reported in other studies. Comparison of expression of IL-17A with IFN-γ revealed subsets co-expressed IL-17A and IFN-γ in cattle, sheep, and goats. The new mAb expands opportunities for immunology research in ruminants and swine.

Keywords: Cattle; Goat; IL-17A; Monoclonal antibody; Sheep; Swine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Flow Cytometry / methods
Goats
Interleukin-17 / immunology*
Receptors, Antigen, T-Cell, gamma-delta / immunology*
Ruminants
Sheep
Swine
T-Lymphocyte Subsets / immunology*

Substances

Interleukin-17
Receptors, Antigen, T-Cell, gamma-delta