Real-time analysis of the cytopathic effect of African swine fever virus

Galina Burmakina; Kirill Bliznetsov; Alexander Malogolovkin

doi:10.1016/j.jviromet.2018.04.003

Real-time analysis of the cytopathic effect of African swine fever virus

J Virol Methods. 2018 Jul:257:58-61. doi: 10.1016/j.jviromet.2018.04.003. Epub 2018 Apr 5.

Authors

Galina Burmakina¹, Kirill Bliznetsov², Alexander Malogolovkin³

Affiliations

¹ Federal Research Center for Virology and Microbiology (FRCVM), 601125, Volginskiy, Bakulova 1, Russia.
² LLC "Alamed", Moskva, Russia.
³ Federal Research Center for Virology and Microbiology (FRCVM), 601125, Volginskiy, Bakulova 1, Russia. Electronic address: Malogolovkin@inbox.ru.

PMID: 29627336
DOI: 10.1016/j.jviromet.2018.04.003

Abstract

Conventional methods, which quantitatively assess virus replication, are based on direct examination of viral cytopathic effect (CPE), which is time consuming, tedious and based on endpoint reading. The Real-Time Cell Analysis (RTCA) xCELLigence® system offers an alternative approach to evaluate virus-induced CPE, and here was evaluated as a means to dynamically assess CPE caused by African swine fever virus (ASFV). RTCA was used to identify optimum time for ASFV infection based on cell index (CI) and to evaluate ASFV CPE kinetics in COS-1 cells. Data indicated that the RTCA has tremendous potential to methodologically and quantitatively improve assays used to study efficiency of ASFV drug inhibitors and neutralizing antibodies.

Keywords: African swine fever virus; Real-time cell analysis system.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

African Swine Fever Virus / growth & development*
Animals
COS Cells
Chlorocebus aethiops
Cytological Techniques / methods*
Cytopathogenic Effect, Viral*
Time Factors
Viral Load / methods*
Virus Replication*