NGS combined with phylogenetic analysis to detect HIV-1 dual infection in Romanian people who inject drugs

Microbes Infect. 2018 May;20(5):308-311. doi: 10.1016/j.micinf.2018.03.004. Epub 2018 Apr 4.

Abstract

Dual HIV infections are possible and likely in people who inject drugs (PWID). Thirty-eight newly diagnosed patients, 19 PWID and 19 heterosexually HIV infected were analyzed. V2V3 loop of HIV-1 env gene was sequenced on the NGS platform 454 GSJunior (Roche). HIV-1 dual/multiple infections were identified in five PWID. For three of these patients, the reconstructed variants belonged to pure F1 subtype and CRF14_BG strains according to phylogenetic analysis. New recombinant forms between these parental strains were identified in two PWID samples. NGS data can provide, with the help of phylogenetic analysis, important insights about the intra-host sub-population structure.

Keywords: HIV dual infection; Next generation sequencing; People who inject drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Users*
  • Genetic Variation
  • Genome, Viral / genetics
  • Genotype
  • HIV Infections / blood
  • HIV Infections / complications*
  • HIV Infections / virology*
  • HIV-1 / classification
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Phylogeny
  • RNA, Viral / genetics
  • Reassortant Viruses / classification
  • Reassortant Viruses / genetics
  • Reassortant Viruses / isolation & purification
  • Romania
  • Sequence Analysis, DNA
  • Substance Abuse, Intravenous / virology
  • Superinfection / blood
  • Superinfection / virology*
  • env Gene Products, Human Immunodeficiency Virus / genetics

Substances

  • RNA, Viral
  • env Gene Products, Human Immunodeficiency Virus